外壳蛋白RNA介导的转基因烟草对安第斯马铃薯斑驳病毒的抗性

Coat protein RNAs-mediated protection against Andean potato mottle virus in transgenic tobacco.

作者信息

Neves-Borges A C., Collares W M., Pontes J A., Breyne P, Farinelli L, de Oliveira D E.

机构信息

Laboratório de Genética Molecular Vegetal, Departamento de Genética, Instituto de Biologia, CCS, UFRJ, CEP 21944-970, RJ, Rio de Janeiro, Brazil

出版信息

Plant Sci. 2001 Mar;160(4):699-712. doi: 10.1016/s0168-9452(00)00452-0.

DOI:10.1016/s0168-9452(00)00452-0

PMID:11448745

Abstract

The expression of translatable sequences of either one of the two Andean potato mottle virus (APMoV) coat protein (CP) genes (CP22 and CP42) and of the nontranslatable sequence of CP42 in transgenic tobacco provided protection against APMoV. Resistance was mediated by CP transgene RNAs rather than the protein, as an inverse correlation between resistance and the accumulation levels of CPs transgene mRNAs was observed. These data indicated that a post-transcriptional gene silencing (PTGS) mechanism is likely involved in the APMoV CP RNA-mediated protection. Moreover, the HindIII-AccI restriction pattern of the CP22 transgene was different in susceptible and resistant transgenic plants, suggesting the involvement of methylation in PTGS. Southern blot experiments also revealed that CPs transgene insertion loci and organisation in the plant genome may play a role in determining the degree of protection.

摘要

两种安第斯马铃薯斑驳病毒（APMoV）外壳蛋白（CP）基因（CP22和CP42）中任一基因的可翻译序列以及CP42的不可翻译序列在转基因烟草中的表达赋予了对APMoV的抗性。抗性是由CP转基因RNA介导的，而非蛋白质，因为观察到抗性与CP转基因mRNA的积累水平呈负相关。这些数据表明，转录后基因沉默（PTGS）机制可能参与了APMoV CP RNA介导的保护作用。此外，CP22转基因的HindIII-AccI限制性图谱在敏感和抗性转基因植物中有所不同，这表明甲基化参与了PTGS。Southern杂交实验还表明，CP转基因在植物基因组中的插入位点和组织方式可能在决定保护程度方面发挥作用。