Kretowski A, Kinalska I
Klinika Endokrynologii AM ul. M. Curie-Sklodowskiej 24 a 15-276 Białystok.
Przegl Lek. 2001;58(1):16-9.
There is an increasing evidence that T helper lymphocytes (CD4+) play a key role in the etiopathogenesis of type 1 diabetes. The aim of the present study was to evaluate the presence of T helper lymphocyte subpopulations: naive (CD4+CD45RA+), memory cells (CD4+CDRO+) and lymphocytes coexpressing both studied phenotypes (CD4+CD45RA+CD45RO+) in subjects at risk of type 1 diabetes (first degree relatives of IDDM patients with autoantibodies) in comparison to age and sex matched controls. We observed higher percentages of lymphocytes coexpressing CD45RA and CD45RO antigens in peripheral blood of first degree relatives with "pro-diabetogenic" DRB10401 allele and/or with impairment of first phase of insulin release (FPIR) in IVGTT. The CD4+CD45RA+/CD4+CD45RO+ cells ratio was significantly lower in subjects with protective DQB10602 alelle and/or higher FPIR levels. The alterations of CD45RA and CD45RO antigens expression on T helper cells in prediabetics suggest the significant role of naive or/and memory CD4+ T cell subsets in the pathogenesis of diabetes type 1. It could be suggested that CD4+CD45RA+/CD4+CD45RO+ ratio could serve as surrogate marker of diabetes type 1 risk development and presumably for estimation of the efficacy of the preventive procedures in subjects at high risk of IDDM, but further prospective studies are needed.
越来越多的证据表明，辅助性T淋巴细胞（CD4+）在1型糖尿病的发病机制中起关键作用。本研究的目的是评估1型糖尿病高危人群（患有自身抗体的IDDM患者的一级亲属）与年龄和性别匹配的对照组相比，辅助性T淋巴细胞亚群的存在情况：初始T细胞（CD4+CD45RA+）、记忆细胞（CD4+CD45RO+）以及同时表达这两种研究表型的淋巴细胞（CD4+CD45RA+CD45RO+）。我们观察到，携带“促糖尿病发生”的DRB10401等位基因和/或静脉葡萄糖耐量试验（IVGTT）中胰岛素释放第一相受损的一级亲属外周血中，同时表达CD45RA和CD45RO抗原的淋巴细胞百分比更高。携带保护性DQB10602等位基因和/或胰岛素释放第一相水平较高的受试者中，CD4+CD45RA+/CD4+CD45RO+细胞比值显著更低。糖尿病前期患者辅助性T细胞上CD45RA和CD45RO抗原表达的改变表明，初始或/和记忆性CD4+T细胞亚群在1型糖尿病发病机制中起重要作用。可以认为，CD4+CD45RA+/CD4+CD45RO+比值可作为1型糖尿病风险发展的替代标志物，并可能用于评估IDDM高危人群预防措施的疗效，但还需要进一步的前瞻性研究。
