白内障组织后晶状体的免疫细胞化学特征——信号分子（生长因子、细胞因子、其他信号分子）、细胞骨架蛋白、细胞和细胞外基质蛋白。

Immunocytochemical features of lens after cataract tissue--signalling molecules (growth factors, cytokines, other signalling molecules), cytoskeleton proteins, cellular and extracellular matrix proteins.

作者信息

Shigemitsu T, Ishiguro K, Shimizu Y, Horiguchi M, Kasahara M, Arakaki S

机构信息

Department of Ophthalmology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.

出版信息

Int Ophthalmol. 1999;23(3):137-44. doi: 10.1023/a:1010640118852.

DOI:10.1023/a:1010640118852

PMID:11456250

Abstract

UNLABELLED

We examined the immunocytochemical features of after cataract tissue and cataractous lens epithelium prior to cataract surgery and at the time of surgery.

METHODS

This study included 23 cases. Antibodies against the following antigens were used: epidermal growth factor (EGF), EGF receptor (EGF-R), fibroblast growth factor (FGF), FGF receptor (FGF-R), transforming growth factor (TGF)-beta, TGF-beta receptor II (TGF-beta -RII), insulin-like growth factor (IGF)II, platelet derived growth factor (PDGF)-AB, interleukin (IL)-6, IL-1 receptor II, tumor necrosis factor (TNF)-alpha, tissue plasminogen activator (TPA), prostaglandin (PG) E2, plasminogen activator inhibitor (PAI)-1, alpha smooth muscle actin (alpha-SMA), keratin, vimentin, myosin, fibronectin, laminin, types I, II, III, IV, V and VI collagen (Col), and lens epithelial cells (LECs).

RESULTS

The following substances were present in posterior and anterior capsular fibrosis of after cataract: EGF, EGF-R, FGF, FGF-R, TGF-beta, TGF-beta-RII, IGF-II, PDGF-AB, IL-6, IL-1RII, TNF-alpha, PGE2, PAI-1, alpha-SMA, keratin, fibronectin, laminin, Type I col, Type II to VI col and LECs. Elschnig's pearls of after cataract expressed FGF-R, TNF-alpha, laminin and LECs. Soemmering's ring of after cataract tissue expressed EGF, FGF, FGF-R, IL-1RII, TPA, PAl-1, keratin and LECs. The following substances were present in cataractous lens epithelium prior to cataract surgery: EGF, EGF-R, FGF, FGF-R, TGF-beta-RII, IL-1-RII, TNF-alpha, PAI-1, keratin, laminin and LECs. The following substances were present in the lens epithelium of anterior capsules collected at the time of cataract surgery: EGF, EGF-R, FGF, FGF-R, TGF-beta, TGF-beta-RII, IGF-II, IL-6, IL-1-RII, TNF-alpha, PGE2, keratin, fibronectin, laminin, Type IV Col and LECs.

CONCLUSIONS

These results prove that many kinds of signalling molecules and proteins exist in the process of after cataract formation.

摘要

未标注

我们研究了后发性白内障组织以及白内障手术前和手术时的白内障晶状体上皮的免疫细胞化学特征。

方法

本研究纳入23例病例。使用了针对以下抗原的抗体：表皮生长因子（EGF）、表皮生长因子受体（EGF-R）、成纤维细胞生长因子（FGF）、成纤维细胞生长因子受体（FGF-R）、转化生长因子（TGF）-β、TGF-β受体II（TGF-β-RII）、胰岛素样生长因子（IGF）II、血小板衍生生长因子（PDGF）-AB、白细胞介素（IL）-6、IL-1受体II、肿瘤坏死因子（TNF）-α、组织纤溶酶原激活物（TPA）、前列腺素（PG）E2、纤溶酶原激活物抑制剂（PAI）-1、α平滑肌肌动蛋白（α-SMA）、角蛋白、波形蛋白、肌球蛋白、纤连蛋白、层粘连蛋白、I、II、III、IV、V和VI型胶原（Col）以及晶状体上皮细胞（LECs）。

结果

后发性白内障的后囊膜和前囊膜纤维化中存在以下物质：EGF、EGF-R、FGF、FGF-R、TGF-β、TGF-β-RII、IGF-II、PDGF-AB、IL-6、IL-1RII、TNF-α、PGE2、PAI-1、α-SMA、角蛋白、纤连蛋白、层粘连蛋白、I型胶原、II至VI型胶原和LECs。后发性白内障的Elschnig珠表达FGF-R、TNF-α、层粘连蛋白和LECs。后发性白内障组织的Soemmering环表达EGF、FGF、FGF-R、IL-1RII、TPA、PAI-1、角蛋白和LECs。白内障手术前白内障晶状体上皮中存在以下物质：EGF、EGF-R、FGF、FGF-R、TGF-β-RII、IL-1-RII、TNF-α、PAI-1、角蛋白、层粘连蛋白和LECs。白内障手术时收集的前囊膜晶状体上皮中存在以下物质：EGF、EGF-R、FGF、FGF-R、TGF-β、TGF-β-RII、IGF-II、IL-6、IL-1-RII、TNF-α、PGE2、角蛋白、纤连蛋白、层粘连蛋白、IV型胶原和LECs。