Stevens J, Wall R
School of Biological Sciences, University of Exeter, Exeter EX4 4PS, England, UK.
Forensic Sci Int. 2001 Aug 15;120(1-2):116-23. doi: 10.1016/s0379-0738(01)00417-0.
Phylogenetic relationships among blowfly (Calliphoridae) species of forensic importance are explored using DNA sequence data from the large sub-unit (lsu, 28S) ribosomal RNA (rRNA) gene, the study includes representatives of a range of calliphorid species commonly encountered in forensic analysis in Britain and Europe. The data presented provide a basis to define molecular markers, including the identification of highly informative intra-sequence regions, which may be of use in the identification of larvae for forensic entomology. Phylogenetic analysis of the sequences also provides new insights into the different evolutionary patterns apparent within the family Calliphoridae which, additionally, can provide a measure of the degree of genetic variation likely to be encountered within taxonomic groups of differing forensic utility.
利用来自核糖体RNA(rRNA)基因大亚基(lsu,28S)的DNA序列数据,探讨了具有法医学重要性的丽蝇科(Calliphoridae)物种之间的系统发育关系。该研究包括在英国和欧洲法医分析中常见的一系列丽蝇科物种的代表。所呈现的数据为定义分子标记提供了基础,包括识别高度信息丰富的序列内区域,这些区域可能用于法医昆虫学中幼虫的鉴定。对这些序列的系统发育分析还为丽蝇科内明显的不同进化模式提供了新的见解,此外,还可以衡量在具有不同法医效用的分类群中可能遇到的遗传变异程度。