Masuda A, Sukegawa T, Tani H, Miyamoto T, Sasai K, Morikawa Y, Baba E
Veterinary Internal Medicine, Department of Veterinary Medicine, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Sakai, Japan.
J Vet Med Sci. 2001 Jun;63(6):667-9. doi: 10.1292/jvms.63.667.
To investigate the predominance of Malassezia pachydermatis (M. pachydermatis) as a causative agent of canine otitis externa, ear cerumen samples were observed for adhesion of M. pachydermatis to the cornified epithelial cells by light and electron microscopes. The yeasts appeared not to adhere to the cornified epithelial cells directly, but they seemed to exist in the proximity of the epithelial cells with an electron opaque halo-like space around them. Polysaccharide and lipid staining techniques were conducted to identify the substances existing in that space. Lipid substances, not saccharides, were observed around the yeasts and the cornified epithelial cells. These results suggested that in the canine ear canal malassezia yeast attachment to the cornified epithelial cells is mediated by lipids.
为了研究厚皮马拉色菌（M. pachydermatis）作为犬外耳炎病原体的优势情况，通过光学显微镜和电子显微镜观察耳垢样本中厚皮马拉色菌与角质化上皮细胞的黏附情况。酵母似乎不直接黏附于角质化上皮细胞，而是存在于上皮细胞附近，其周围有一个电子不透明的晕状空间。采用多糖和脂质染色技术来鉴定该空间中存在的物质。在酵母和角质化上皮细胞周围观察到的是脂质物质，而非糖类。这些结果表明，在犬耳道中，马拉色菌酵母与角质化上皮细胞的附着是由脂质介导的。
