Configuration and racemization determination of cysteine residues in peptides by chiral derivatization and HPLC: application to oxytocin peptides.
作者信息
Szabó S, Szókán G, Khlafulla A M, Almás M, Kiss C, Rill A, Schön I
机构信息
Department of Organic Chemistry, Eötvös University, Budapest, Hungary.
出版信息
J Pept Sci. 2001 Jun;7(6):316-22. doi: 10.1002/psc.325.
DOI:10.1002/psc.325
PMID:11461045
Abstract
An improved RP-HPLC method was developed for the determination of the configuration and stereochemical purity of cysteine residues in peptides. The method consists of oxidation of cysteine and cystine residues to cysteic acid, followed by hydrolysis and pre-column chiral derivatization with Val-Marfey's reagent.
摘要
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