小鼠精子中磷脂酶Cβ1基因突变在顶体反应、受精及胚胎发育中的生物学意义。
The biological significance of phospholipase C beta 1 gene mutation in mouse sperm in the acrosome reaction, fertilization, and embryo development.
作者信息
Choi D, Lee E, Hwang S, Jun K, Kim D, Yoon B K, Shin H S, Lee J H
机构信息
Department of Obstetrics and Gynecology, Samsung Medical Center, Sungkyunkwan University School of Medicine, IVF Clinic, Samsung Medical Center, Seoul, Korea.
出版信息
J Assist Reprod Genet. 2001 May;18(5):305-10. doi: 10.1023/a:1016622519228.
PURPOSE
We carried out this study to evaluate the biological significance of phospholipase C beta 1 gene mutation in mouse sperm in the acrosome reaction, fertilization, and embryo development.
METHODS
Study subjects were divided into two groups according to the sperm [intact phospholipase C (PLC) beta 1 and PLC beta 1-/- C57BL/6J x CBA F1 mouse sperm] used. The positive acrosome reaction rate labeled with fluorescein isothiocyanate-Pisum sativum agglutinin, the fertilization rate, and the rate of embryos developed to the stage of morula or blastocyst in the two groups were compared.
RESULTS
The mouse sperm null for the PLC beta 1 gene showed a lower acrosome reaction rate than control sperm (69.2 vs 50.9%, P < 0.05). And the fertilization rate and the rate of embryos developed to the stage of morula or blastocyst were also lower in the group using PLC beta 1-/- mouse sperm compared to the intact group (P < 0.05; 73.5 vs 51.8% and 15.7 vs 4.3%, respectively).
CONCLUSIONS
Mutation of the PLC beta 1 gene in the mouse sperm reduces the acrosome reaction rate, fertilization rate, and embryo development rate, which may be the etiologic factors responsible for the low reproductive rate of PLC beta 1-/- mouse.
目的
我们开展本研究以评估小鼠精子中磷脂酶Cβ1基因突变在顶体反应、受精及胚胎发育中的生物学意义。
方法
根据所使用的精子（完整磷脂酶C（PLC）β1和PLCβ1 - / - C57BL/6J×CBA F1小鼠精子）将研究对象分为两组。比较两组中用异硫氰酸荧光素 - 豌豆凝集素标记的顶体反应阳性率、受精率以及发育至桑葚胚或囊胚阶段的胚胎率。
结果
PLCβ1基因缺失的小鼠精子顶体反应率低于对照精子（69.2%对50.9%，P < 0.05）。与完整组相比，使用PLCβ1 - / - 小鼠精子的组中受精率以及发育至桑葚胚或囊胚阶段的胚胎率也较低（P < 0.05；分别为73.5%对51.8%和15.7%对4.3%）。
结论
小鼠精子中PLCβ1基因突变降低了顶体反应率、受精率及胚胎发育率，这可能是PLCβ1 - / - 小鼠繁殖率低的病因。
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