用来自拉斯穆森脑炎患者的抗GluR3 IgG对大鼠神经元进行标记。

Labeling of rat neurons by anti-GluR3 IgG from patients with Rasmussen encephalitis.

作者信息

Frassoni C, Spreafico R, Franceschetti S, Aurisano N, Bernasconi P, Garbelli R, Antozzi C, Taverna S, Granata T, Mantegazza R

机构信息

Department of Experimental Neurophysiology and Epileptology, Istituto Nazionale Neurologico Carlo Besta, Milan, Italy.

出版信息

Neurology. 2001 Jul 24;57(2):324-7. doi: 10.1212/wnl.57.2.324.

DOI:10.1212/wnl.57.2.324

PMID:11468321

Abstract

The authors report the immunocytochemical localization in rat brain of affinity-purified anti-GluR3 (glutamate receptor) antibodies from two patients with Rasmussen encephalitis (RE) and from immunized rabbits. The distribution of immunolabeling was similar using antibodies from rabbits and patients with RE. No electrophysiologic responses were elicited from acutely dissociated kainate-responsive neurons isolated from rat brain when these antibodies were applied. These findings show that anti-GluR3 antibodies purified from patients with RE bind to specific regions of the CNS but do not act through an excitotoxic mechanism.

摘要

作者报告了从两名患有拉斯穆森脑炎（RE）的患者以及免疫后的兔子体内亲和纯化得到的抗GluR3（谷氨酸受体）抗体在大鼠脑中的免疫细胞化学定位。使用来自兔子和RE患者的抗体时，免疫标记的分布相似。当应用这些抗体时，从大鼠脑急性分离的对红藻氨酸盐有反应的神经元未引发电生理反应。这些发现表明，从RE患者纯化得到的抗GluR3抗体与中枢神经系统的特定区域结合，但并非通过兴奋性毒性机制起作用。