Bezold G, Lange M, Gethöffer K, Pillekamp H, Reindl H, Richter C, Schönian G, Weber L, Peter R U
Department of Dermatology, University of Ulm, Germany.
Eur J Clin Microbiol Infect Dis. 2001 Jun;20(6):421-4. doi: 10.1007/s100960100519.
A competitive polymerase chain reaction (PCR) followed by enzyme-linked-immunoassay-based verification of PCR products has been developed, which facilitated the diagnosis of leishmaniasis in two German soldiers who underwent survival training in the jungle of French Guiana and returned with therapy-resistant pyoderma-like lesions. After treatment with liposomal amphotericin B, the skin manifestations disappeared, and leishmania DNA could no longer be detected by PCR. In the context of growing military involvement in areas where leishmaniasis is prevalent, this assay may help detect or, due to its internal controls, exclude cases of infection with this parasite.
一种竞争性聚合酶链反应（PCR），随后基于酶联免疫测定对PCR产物进行验证，已经被开发出来，这有助于诊断两名在法属圭亚那丛林接受生存训练后回来并患有抗治疗性脓疱样病变的德国士兵的利什曼病。在用脂质体两性霉素B治疗后，皮肤表现消失，PCR检测不到利什曼原虫DNA。鉴于军队在利什曼病流行地区的参与日益增加，该检测方法可能有助于检测，或者由于其内部对照，排除感染这种寄生虫的病例。
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