黄嘌呤脱氢酶/黄嘌呤氧化酶作为大鼠潘氏细胞锌结合蛋白的鉴定。

Identification of xanthine dehydrogenase/xanthine oxidase as a rat Paneth cell zinc-binding protein.

作者信息

Morita Y, Sawada M, Seno H, Takaishi S, Fukuzawa H, Miyake N, Hiai H, Chiba T

机构信息

Department of Internal Medicine, Graduate School of Medicine, Kyoto University, Japan.

出版信息

Biochim Biophys Acta. 2001 Jul 25;1540(1):43-9. doi: 10.1016/s0167-4889(01)00118-5.

DOI:10.1016/s0167-4889(01)00118-5

PMID:11476893

Abstract

Paneth cells are zinc-containing cells localized in small intestinal crypts, but their function has not been fully elucidated. Previously, we showed that an intravenous injection of diphenylthiocarbazone (dithizone), a zinc chelator, induced selective killing of Paneth cells, and purified a zinc-binding protein in Paneth cells. In the present study, we further characterized one of these proteins, named zinc-binding protein of Paneth cells (ZBPP)-1. Partial amino acid sequences of ZBPP-1 showed identity with rat xanthine dehydrogenase (XD)/xanthine oxidase (XO). Anti-rat XD antibody (Ab) recognized ZBPP-1, and conversely anti ZBPP-1 Ab recognized 85 kDa fragment of rat XD in Western blotting. Messenger RNA and protein levels of XD were consistent with our previous data on the fluctuation of Paneth cell population after dithizone injection. Thus, ZBPP-1 is an 85 kDa fragment of XD/XO in Paneth cells. XD/XO in Paneth cells may play important roles in intestinal function.

摘要

潘氏细胞是位于小肠隐窝的含锌细胞，但其功能尚未完全阐明。此前，我们发现静脉注射锌螯合剂二苯基硫代卡巴腙（双硫腙）可诱导潘氏细胞选择性死亡，并纯化了潘氏细胞中的一种锌结合蛋白。在本研究中，我们进一步对其中一种名为潘氏细胞锌结合蛋白（ZBPP）-1的蛋白进行了表征。ZBPP-1的部分氨基酸序列与大鼠黄嘌呤脱氢酶（XD）/黄嘌呤氧化酶（XO）相同。抗大鼠XD抗体（Ab）可识别ZBPP-1，相反，在蛋白质印迹法中，抗ZBPP-1抗体可识别大鼠XD的85 kDa片段。XD的信使核糖核酸和蛋白质水平与我们之前关于双硫腙注射后潘氏细胞群体波动的数据一致。因此，ZBPP-1是潘氏细胞中XD/XO的85 kDa片段。潘氏细胞中的XD/XO可能在肠道功能中发挥重要作用。