Specht B U, Wahl M, Kolb H J, Brendel W
Arch Int Pharmacodyn Ther. 1975 Feb;213(2):242-50.
Kallikrein covalently attached to polyvinylpyrrolidone was prepared. The kinetic parameters, the molecular weight and the blood pressure depressor effect of kallikrein and kallikrein bound to polyvinylpyrrolidone are compared. Analytical ultracentrifugation showed a mean molecular weight of 110 000 dalton for kallikrein bound to polyvinylpyrrolidone. The specific activity of kallikrein-PVP was 4.6 times lower when compared with native kallikrein, however K-M was only slightly different. The maximal reduction and the duration of the decrease of mean arterial blood pressure were statistically not different from each other when kallikrein and kallikrein-PVP were injected in amounts of the same esterolytic activity.
制备了共价连接到聚乙烯吡咯烷酮上的激肽释放酶。比较了激肽释放酶以及与聚乙烯吡咯烷酮结合的激肽释放酶的动力学参数、分子量和降压效果。分析超速离心显示，与聚乙烯吡咯烷酮结合的激肽释放酶的平均分子量为110000道尔顿。与天然激肽释放酶相比，激肽释放酶-聚乙烯吡咯烷酮的比活性低4.6倍，然而米氏常数仅略有不同。当以相同酯解活性的量注射激肽释放酶和激肽释放酶-聚乙烯吡咯烷酮时，平均动脉血压的最大降低幅度和降低持续时间在统计学上没有差异。
