帕克斯7（Pax7）转录本在肌肉和神经细胞的决定中起关键作用。

A key role for Pax7 transcripts in determination of muscle and nerve cells.

作者信息

Ziman M R, Thomas M, Jacobsen P, Beazley L

机构信息

Department of Zoology, University of Western Australia, Nedlands, Perth, Western Australia 6907, Australia.

出版信息

Exp Cell Res. 2001 Aug 15;268(2):220-9. doi: 10.1006/excr.2001.5282.

DOI:10.1006/excr.2001.5282

PMID:11478848

Abstract

Embryonic teratocarcinoma mouse cells (P19) and embryonic NIH3T3 fibroblasts were induced chemically to differentiate along neurogenic or myogenic lineages. The expression profiles of Pax7 alternate transcripts were then assessed by RNA isolation and RT-PCR. Only two transcripts, Pax7b and Pax7d, were expressed in the neurogenic lineage. By contrast, in adult skeletal muscle, four transcripts, Pax7a-d, were expressed in the myogenic lineage. Moreover, P19 cells were shown to undergo neural cell differentiation when stably transfected with a single Pax7 transcript, PAX7b, generated from human skeletal muscle. Our results suggest a key role for Pax7 transcripts in lineage determination.

摘要

胚胎性畸胎癌小鼠细胞（P19）和胚胎NIH3T3成纤维细胞通过化学诱导沿着神经源性或肌源性谱系分化。然后通过RNA分离和逆转录聚合酶链反应（RT-PCR）评估Pax7可变转录本的表达谱。在神经源性谱系中仅表达两种转录本，即Pax7b和Pax7d。相比之下，在成年骨骼肌中，肌源性谱系中表达四种转录本，即Pax7a-d。此外，当用源自人类骨骼肌的单个Pax7转录本PAX7b稳定转染时，P19细胞显示出经历神经细胞分化。我们的结果表明Pax7转录本在谱系确定中起关键作用。