聚合酶链反应检测根管内牙髓感染中的齿垢密螺旋体。
Polymerase chain reaction detection of Treponema denticola in endodontic infections within root canals.
作者信息
Siqueira J F, Rôças I N, Favieri A, Oliveira J C, Santos K R
机构信息
Department of Endodontics, Faculty of Dentistry, Estácio de Sá University, Rio de Janeiro, Brazil.
出版信息
Int Endod J. 2001 Jun;34(4):280-4. doi: 10.1046/j.1365-2591.2001.00382.x.
AIM
A 16S rDNA-based polymerase chain reaction (PCR) method was used to detect the occurrence of Treponema denticola in root canals.
METHODOLOGY
Samples were collected from 54 single-rooted teeth having carious lesions and necrotic pulps. DNA extracted from the samples was amplified using the PCR assay, which yielded a specific fragment of T. denticola 16S rDNA.
RESULTS
T. denticola was detected in 10 of 29 asymptomatic cases (34.5%), eight of 15 root canals that were tender to percussion (53.3%), and five of 10 teeth with acute periradicular abscess (50%). In general, T. denticola was found in 23 of 54 cases (42.6%).
CONCLUSIONS
Findings suggest that T. denticola can be involved in the pathogenesis of periradicular lesions of endodontic origin.
目的
采用基于16S rDNA的聚合酶链反应（PCR）方法检测根管中齿垢密螺旋体的存在情况。
方法
从54颗患有龋损和牙髓坏死的单根牙采集样本。使用PCR检测法对从样本中提取的DNA进行扩增，该检测法产生了齿垢密螺旋体16S rDNA的特定片段。
结果
在29例无症状病例中的10例（34.5%）、15例叩诊疼痛的根管中的8例（53.3%）以及10例急性根尖周脓肿患牙中的5例（50%）检测到齿垢密螺旋体。总体而言，54例病例中有23例（42.6%）发现齿垢密螺旋体。
结论
研究结果表明齿垢密螺旋体可能参与牙髓源性根尖周病变的发病机制。
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