[大鼠垂体促性腺激素的等电聚焦]

[Isoelectric focusing of rat pituitary gonadotropins].

作者信息

Kercret H, Duval J

出版信息

Biochimie. 1975;57(1):85-90. doi: 10.1016/s0300-9084(75)80113-1.

Abstract

Isoelectric focusing of rat gonadotropins has been studied using a small scale column and various pH gradients. Hormones were detected by radioimmunoassay. FSH focuses as a single peak, the pI being 2.8. It is thus slightly more acidic than the pI of FSH from other species. LH is more heterogeneous, the main activity focusing in the pH 9.0 area, whereas a second activity appears, for some samples, in the acidic part of the gradient. TSH exhibits a broad zone of activity between pH 7.0 and 10.0. The fractionation of pituitary glycoproteins using a pH 3-10 gradient followed by removal of sucrose and ampholytes through Sephadex G 50 chromatography allows the recovery with good yields of a purified rat FSH fraction devoid of LH activity as estimated by radioimmunoassay.

摘要

利用小型柱和各种pH梯度对大鼠促性腺激素进行了等电聚焦研究。通过放射免疫测定法检测激素。促卵泡激素（FSH）聚焦为单一峰，其等电点（pI）为2.8。因此，它比其他物种的FSH的pI略酸性更强。促黄体生成素（LH）更具异质性，主要活性聚焦在pH 9.0区域，而对于一些样品，第二种活性出现在梯度的酸性部分。促甲状腺激素（TSH）在pH 7.0至10.0之间表现出较宽的活性区。使用pH 3 - 10梯度对垂体糖蛋白进行分级分离，然后通过葡聚糖凝胶G 50色谱法去除蔗糖和两性电解质，通过放射免疫测定法估计，可高收率回收不含LH活性的纯化大鼠FSH组分。