Furuya M, Ishikura H, Ogawa Y, Kawarada Y, Shibata M, Fujimoto S, Yoshiki T
Department of Pathology, Hokkaido University School of Medicine, N-15, W-7, Kita-ku, Sapporo 060-8638, Japan.
Pathobiology. 2000;68(6):239-44. doi: 10.1159/000055932.
Expression of matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs) in serous tumors of the ovary was investigated to determine whether and how these proteolytic enzymes are associated with the progression of these tumors.
Cyst fluid of 24 serous ovarian tumors (8 adenocarcinomas, 2 borderline tumors and 14 adenomas) was analyzed using gelatin/casein zymography and enzyme-linked immunosorbent assay.
Concentrations of MMP-9 and MMP-2 were statistically higher in serous adenocarcinomas than in serous adenomas (p < 0.01, p < 0.05, respectively), while the concentrations of TIMP-1 and TIMP-2 showed no significant difference between adenocarcinomas and adenomas. The molar ratio of TIMP-2/MMP-2 was lower in adenocarcinomas than in adenomas (p < 0.05). With gelatin zymography, the MMP-9 band was detected in all serous adenocarcinomas, but only in 8 of 14 serous adenomas (p = 0.05). Using casein zymography, MMP-7 was more frequently detected in serous adenocarcinomas (7/8) than in serous adenomas (4/14; p < 0.05).
These observations indicate that matriolytic enzymes such as MMP-2, MMP-7 and MMP-9 are secreted into cyst fluid from serous adenocarcinoma tissues. In part, the aggressive invasion of serous carcinoma cells may be explained by the expression of matriolytic enzymes.
研究基质金属蛋白酶（MMPs）及其组织抑制剂（TIMPs）在卵巢浆液性肿瘤中的表达，以确定这些蛋白水解酶是否以及如何与这些肿瘤的进展相关。
采用明胶/酪蛋白酶谱法和酶联免疫吸附测定法分析24例卵巢浆液性肿瘤（8例腺癌、2例交界性肿瘤和14例腺瘤）的囊液。
浆液性腺癌中MMP-9和MMP-2的浓度在统计学上高于浆液性腺瘤（分别为p < 0.01，p < 0.05），而TIMP-1和TIMP-2的浓度在腺癌和腺瘤之间无显著差异。腺癌中TIMP-2/MMP-2的摩尔比低于腺瘤（p < 0.05）。通过明胶酶谱法，在所有浆液性腺癌中均检测到MMP-9条带，但在14例浆液性腺瘤中仅8例检测到（p = 0.05）。采用酪蛋白酶谱法，浆液性腺癌（7/8）中MMP-7的检出频率高于浆液性腺瘤（4/14；p < 0.05）。
这些观察结果表明，诸如MMP-2、MMP-7和MMP-9等基质溶解酶从浆液性腺癌组织分泌到囊液中。浆液性癌细胞的侵袭性在一定程度上可能由基质溶解酶的表达来解释。
