用布鲁氏菌流产疫苗RB51株对性成熟猪进行口服接种。
Oral vaccination of sexually mature pigs with Brucella abortus vaccine strain RB51.
作者信息
Edmonds M D, Samartino L E, Hoyt P G, Hagius S D, Walker J V, Enright F M, Schurig G G, Elzer P
机构信息
Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803, USA.
出版信息
Am J Vet Res. 2001 Aug;62(8):1328-31. doi: 10.2460/ajvr.2001.62.1328.
OBJECTIVE
To develop a novel oral vaccine delivery system for swine, using the rough vaccine strain of Brucella abortus.
ANIMALS
56 crossbred pigs from a brucellosis-free facility.
PROCEDURE
In 3 separate experiments, pigs were orally vaccinated with doses of 1 x 10(9) to > 1 x 10(11) CFU of strain RB51 vaccine. The vaccine was placed directly on the normal corn ration, placed inside a whole pecan, or mixed with cracked pecans and corn.
RESULTS
Oral vaccination of pigs with vaccine strain RB51 resulted in a humoral immune response to strain RB51 and short-term colonization of the regional lymph nodes.
CONCLUSIONS AND CLINICAL RELEVANCE
A viscous liquid such as Karo corn syrup in association with pecans that scarify the oral mucosa are necessary when placing the live vaccine directly onto corn or other food rations. Doses of > 1 x 10(11) CFU of RB51 organisms/pig in this mixture ensures 100% colonization of regional lymph nodes via the oral route. This method may allow an efficient and economical means to vaccinate feral swine for brucellosis.
目的
利用流产布鲁氏菌粗糙疫苗株开发一种新型猪用口服疫苗递送系统。
动物
来自无布鲁氏菌病设施的56头杂交猪。
程序
在3个独立实验中，给猪口服接种剂量为1×10⁹至＞1×10¹¹CFU的RB51疫苗株。疫苗直接放置在普通玉米日粮上、置于整个山核桃内或与破碎的山核桃和玉米混合。
结果
用RB51疫苗株对猪进行口服接种可引发对RB51菌株的体液免疫反应以及区域淋巴结的短期定植。
结论及临床意义
将活疫苗直接放置在玉米或其他食物日粮上时，需要一种粘性液体（如卡罗玉米糖浆）并搭配能划破口腔黏膜的山核桃。该混合物中每头猪接种＞1×10¹¹CFU的RB51菌可确保通过口服途径使区域淋巴结100%定植。此方法可能为给野猪接种布鲁氏菌病疫苗提供一种高效且经济的手段。
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