DNA的渗透性质：反离子凝聚理论的批判性评估
Osmotic properties of DNA: critical evaluation of counterion condensation theory.
作者信息
Hansen P L, Podgornik R, Parsegian V A
机构信息
Laboratory of Physical and Structural Biology National Institute of Child Health and Human Development National Institutes of Health, Bethesda, Maryland 20892-5626, USA.
出版信息
Phys Rev E Stat Nonlin Soft Matter Phys. 2001 Aug;64(2 Pt 1):021907. doi: 10.1103/PhysRevE.64.021907. Epub 2001 Jul 23.
The osmotic coefficient of B-DNA in water may, in dilute solutions, deviate by as much as 100% from predictions based on a simple line-charge "counterion condensation" theory. In contrast, a cell model description of the ionic atmosphere near a cylindrical polyelectrolyte predicts osmotic properties that are in surprisingly good harmony with all available experimental findings over a wide range of DNA concentrations. We argue that the neglect of molecular features, such as finite radius, makes line-charge condensation theory inapplicable at all but impractically low polyelectrolyte concentrations.
在稀溶液中，B - DNA在水中的渗透系数可能会与基于简单线电荷“反离子凝聚”理论的预测值相差高达100%。相比之下，圆柱形聚电解质附近离子氛围的细胞模型描述所预测的渗透性质，在很宽的DNA浓度范围内，与所有现有实验结果惊人地吻合。我们认为，忽略诸如有限半径等分子特征，使得线电荷凝聚理论除了在低得不切实际的聚电解质浓度下之外，在其他情况下都不适用。
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