孔蛋白在金电极上的组装。

Assembly of pore proteins on gold electrodes.

作者信息

Hong Q, Terrettaz S, Ulrich W P, Vogel H, Lakey J H

机构信息

School of Biochemistry and Genetics, The Medical School, Newcastle upon Tyne, NE2 4HH, UK.

出版信息

Biochem Soc Trans. 2001 Aug;29(Pt 4):578-82. doi: 10.1042/bst0290578.

DOI:10.1042/bst0290578

PMID:11498031

Abstract

Protein-protein interactions at the cell surface are important in the activity of bacterial toxins such as colicins. We have developed methods to study these events using tethered lipid bilayers, which can be probed by impedance spectroscopy and surface plasmon resonance. Recently we have attached the receptor proteins directly to gold electrodes and this offers new possibilities for measuring protein-protein interactions on solid supports.

摘要

细胞表面的蛋白质-蛋白质相互作用在诸如大肠杆菌素等细菌毒素的活性中起着重要作用。我们已经开发出利用拴系脂质双层来研究这些事件的方法，拴系脂质双层可以通过阻抗谱和表面等离子体共振进行探测。最近，我们已将受体蛋白直接连接到金电极上，这为测量固体支持物上的蛋白质-蛋白质相互作用提供了新的可能性。