气相色谱法测定血清和尿液中的β-苯乙双胍及其代谢产物对羟基-β-苯乙双胍。

Gas chromatographic determination of beta-phenethylbiguanide and its metabolite p-hydroxy-beta-phenethylbiguanide in serum and urine.

作者信息

Mottale M, Stewart C J

出版信息

J Chromatogr. 1975 Mar 26;106(2):263-70. doi: 10.1016/s0021-9673(00)93834-2.

DOI:10.1016/s0021-9673(00)93834-2

Abstract

A gas chromatographic method is presented for the detection of beta-phenethylbiguanide (PEBG) and its metabolite, p-hydroxy-beta-phenethylbiguanide (p-OHPEBG). The procedure is applicable for the determination of the drug and its metabolite in the serum and urine of rats. The detection limit is 0.2 mug PEBG and 0.5 mug p-OH PEBG per ml of serum or urine. A time course study of blood concentration and elimination rate following intraperitoneal injection of 100 mg/kg of PEBG to normal rats was performed. Beta-PEBG was found to be present in the blood and the urine, p-OH PEBG was only detected in the urine. Twenty-four hours following intraperitoneal injection, the urine contained 32% of the administered dose, 20% as unaltered PEBG and 12% as p-OH PEBG.

摘要

本文介绍了一种气相色谱法，用于检测β-苯乙双胍（PEBG）及其代谢物对羟基-β-苯乙双胍（p-OHPEBG）。该方法适用于测定大鼠血清和尿液中的药物及其代谢物。血清或尿液中PEBG的检测限为每毫升0.2微克，p-OH PEBG的检测限为每毫升0.5微克。对正常大鼠腹腔注射100毫克/千克的PEBG后，进行了血药浓度和消除速率的时间进程研究。发现血液和尿液中存在β-PEBG，仅在尿液中检测到p-OH PEBG。腹腔注射24小时后，尿液中含有32%的给药剂量，其中20%为未改变的PEBG，12%为p-OH PEBG。

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