Amikura R, Hanyu K, Kashikawa M, Kobayashi S
Gene Experimental Center, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.
Mech Dev. 2001 Sep;107(1-2):97-104. doi: 10.1016/s0925-4773(01)00455-5.
In Drosophila, polar plasm contains polar granules, which deposit the factors required for the formation of pole cells, germ line progenitors. Polar granules are tightly associated with mitochondria in early embryos, suggesting that mitochondria could contribute to pole cell formation. We have previously reported that mitochondrial large and small rRNAs (mtrRNAs) are transported from mitochondria to polar granules prior to pole cell formation and the large rRNA is essential for pole cell formation. Here we show that the localization of mtrRNAs is diminished in embryos laid by tudor mutant females, although the polar granules are maintained. We also found that Tud protein was colocalized with mtrRNAs at the boundaries between mitochondria and polar granules when the transport of mtrRNAs takes place. These observations suggest that Tud mediates the transport of mtrRNAs from mitochondria to polar granules.
在果蝇中，极质含有极粒，极粒沉积形成极细胞（生殖系祖细胞）所需的因子。在早期胚胎中，极粒与线粒体紧密相连，这表明线粒体可能有助于极细胞的形成。我们之前曾报道，线粒体大、小核糖体RNA（mtrRNAs）在极细胞形成之前从线粒体转运至极粒，且大核糖体RNA对极细胞形成至关重要。在此我们表明，尽管极粒得以维持，但 tudor 突变雌性所产胚胎中 mtrRNAs 的定位减少。我们还发现，当 mtrRNAs 发生转运时，Tud 蛋白在线粒体与极粒之间的边界处与 mtrRNAs 共定位。这些观察结果表明，Tud 介导 mtrRNAs 从线粒体到极粒的转运。
