细胞色素P450 86B1在叶绿体外被膜中的定位。

Localization of CYP86B1 in the outer envelope of chloroplasts.

作者信息

Watson C J, Froehlich J E, Josefsson C A, Chapple C, Durst F, Benveniste I, Coolbaugh R C

机构信息

Department of Botany and Plant Pathology, Purdue University, W. Lafayette, IN 47907, USA.

出版信息

Plant Cell Physiol. 2001 Aug;42(8):873-8. doi: 10.1093/pcp/pce110.

DOI:10.1093/pcp/pce110

PMID:11522915

Abstract

CYP86B1 was cloned from a cDNA library and the protein expressed in E. coli. The protein gave the expected carbon monoxide difference spectrum. Using in vitro import assays with isolated pea chloroplasts, CYP86B1 was shown to be associated with the outer chloroplastic envelope membrane. This study provides the first direct evidence for a chloroplast-localized cytochrome P450-dependent monooxygenase.

摘要

CYP86B1从一个cDNA文库中克隆出来，并在大肠杆菌中表达该蛋白质。该蛋白质呈现出预期的一氧化碳差光谱。通过使用分离的豌豆叶绿体进行体外导入试验，表明CYP86B1与叶绿体的外被膜相关。这项研究为叶绿体定位的细胞色素P450依赖性单加氧酶提供了首个直接证据。