橄榄（油橄榄）中一个新的重复DNA序列家族。

A new repetitive DNA sequence family in the olive (Olea europaea L.).

作者信息

Lorite P, García M F, Carrillo J A, Palomeque T

机构信息

Departamento de Biología Experimental, Area de Genética, Universidad de Jaén, Jaén, Spain.

出版信息

Hereditas. 2001;134(1):73-8. doi: 10.1111/j.1601-5223.2001.00073.x.

DOI:10.1111/j.1601-5223.2001.00073.x

PMID:11525067

Abstract

Two families of repeated DNA sequences were cloned from Olea europaea ssp sativa cv. "Picual". The first repetitive DNA is organized in a tandem repeat of monomers of 178 bp. Sequencing of several clones showed that it is relatively A-T rich (54.49%) and possesses short direct and inverted subrepeats as well as some palindromic sequences. Comparison between the monomers revealed heterogeneity of the sequence primary structure. This repetitive DNA is present in several cultivars of olive cultivates. Comparison of sequences with other repetitive DNAs described in Olea europaea has been carried out. No significant similarity was found. All the obtained results suggest that this repetitive DNA described here is a new family of repetitive DNA. The second repetitive DNA is organized in a tandem repeat of monomers of 78 bp. This second family of repetitive DNA showed significant similarity with other repetitive DNAs previously described in Olea europaea. Their existence in new cultivars of olive is shown.

摘要

从油橄榄（Olea europaea ssp sativa cv. “Picual”）中克隆出了两个重复DNA序列家族。第一个重复DNA由178 bp的单体串联重复组成。对几个克隆进行测序表明，它相对富含A-T（54.49%），具有短的正向和反向亚重复序列以及一些回文序列。单体之间的比较揭示了序列一级结构的异质性。这种重复DNA存在于多个橄榄栽培品种中。已将其序列与油橄榄中描述的其他重复DNA进行了比较。未发现显著相似性。所有获得的结果表明，这里描述的这种重复DNA是一个新的重复DNA家族。第二个重复DNA由78 bp的单体串联重复组成。这第二个重复DNA家族与油橄榄中先前描述的其他重复DNA显示出显著相似性。展示了它们在橄榄新品种中的存在情况。