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一种用于高分辨率光测量的简单光纤微探针:在海洋沉积物中的应用。

A simple fiber-optic microprobe for high resolution light measurements: application in marine sediment.

作者信息

Jorgensen B B, Des Marais D J

机构信息

NASA Ames Research Center, Moffett Field, California 94035, USA.

出版信息

Limnol Oceanogr. 1986;31(6):1376-83. doi: 10.4319/lo.1986.31.6.1376.

Abstract

A fiber-optic microphobe is described which is inexpensive and simple to build and use. It consists of an 80-micrometers optical fiber which at the end is tapered down to a rounded sensing tip of 20-30-micrometers diameter. The detector is a hybrid photodiode/amplifier. The probe has a sensitivity of 0.01 microEinst m-2 s-1 and a spectral range of 300-1,100 nm. Spectral light gradients were measured in fine-grained San Francisco Bay sediment that had an undisturbed diatom coating on the surface. The photic zone of the mud was only 0.4 mm deep. Measured in situ spectra showed extinction maxima at 430-520, 620-630, 670, and 825-850 nm due to absorption by chlorophyll a, carotenoids, phycocyanin, and bacterio-chlorophyll a. Maximum light penetration in the visible range was found in both the violet and the red < or = 400 and > or = 700 nm.

摘要

描述了一种光纤微生物探测器,其造价低廉,制作和使用都很简单。它由一根80微米的光纤组成,光纤末端逐渐变细,形成一个直径为20 - 30微米的圆形传感尖端。探测器是一个混合光电二极管/放大器。该探头的灵敏度为0.01微爱因斯坦·米-2·秒-1,光谱范围为300 - 1100纳米。在旧金山湾细颗粒沉积物中测量了光谱光梯度,沉积物表面有未受干扰的硅藻涂层。泥浆的光合带仅0.4毫米深。现场测量光谱显示,由于叶绿素a、类胡萝卜素、藻蓝蛋白和细菌叶绿素a的吸收,在430 - 520、620 - 630、670和825 - 850纳米处有消光最大值。在可见光范围内,紫光和红光(≤400纳米和≥700纳米)的光穿透率最高。

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