Wooldridge B J, Pineda G, Banuelas-Ornelas J J, Dagda R K, Gasanov S E, Rael E D, Lieb C S
Department of Biological Sciences, The University of Texas at El Paso, 500 West University Avenue, El Paso, TX 79968-0519, USA.
Comp Biochem Physiol B Biochem Mol Biol. 2001 Sep;130(2):169-79. doi: 10.1016/s1096-4959(01)00422-5.
The venom composition of Mojave rattlesnakes (Crotalus scutulatus scutulatus) differs in that some individuals have Mojave toxin and others do not. In order to understand the genetic basis for this difference, genomic DNA samples from Mojave rattlesnakes collected in Arizona, New Mexico, and Texas were analyzed for the presence of DNA sequences that relate to the acidic (Mta) and basic (Mtb) subunits of this toxin. DNA samples were subjected to PCR to amplify nucleotide sequences from second to fourth exons of the acidic and basic subunits. These nucleotide sequences were cloned and sequenced. The nucleotide sequences generated aligned exactly to previously published nucleotide sequences of Mojave toxin. All DNA samples analyzed generated product using the basic subunit primers, and aligned identically to the Mtb nucleotide sequence. However, only 11 out of the 14 samples generated a product with the acidic subunit primers. These 11 sequences aligned identically to the Mta nucleotide sequence. The venom from the three snakes whose DNA did not amplify with the acidic subunit primers were not recognized by antibodies to Mojave toxin. This suggests that snakes with venom lacking Mojave toxin also lack the productive nucleotide sequence for the acidic subunit in their DNA.
莫哈韦响尾蛇（Crotalus scutulatus scutulatus）的毒液成分有所不同，有些个体含有莫哈韦毒素，而有些则没有。为了了解这种差异的遗传基础，对从亚利桑那州、新墨西哥州和得克萨斯州采集的莫哈韦响尾蛇的基因组DNA样本进行了分析，以检测与该毒素的酸性（Mta）和碱性（Mtb）亚基相关的DNA序列的存在情况。对DNA样本进行PCR，以扩增酸性和碱性亚基第二至第四外显子的核苷酸序列。这些核苷酸序列被克隆并测序。所产生的核苷酸序列与先前发表的莫哈韦毒素的核苷酸序列完全一致。所有分析的DNA样本使用碱性亚基引物均产生了产物，并且与Mtb核苷酸序列完全一致。然而，14个样本中只有11个使用酸性亚基引物产生了产物。这11个序列与Mta核苷酸序列完全一致。其DNA不能用酸性亚基引物扩增的三条蛇的毒液不能被抗莫哈韦毒素抗体识别。这表明毒液中缺乏莫哈韦毒素的蛇在其DNA中也缺乏酸性亚基的有效核苷酸序列。
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