一种用于检测肉类和组织中炭疽芽孢杆菌的简单灵敏检测系统。
A simple and sensitive detection system for Bacillus anthracis in meat and tissue.
作者信息
Cheun H I, Makino S I, Watarai M, Shirahata T, Uchida I, Takeshi K
机构信息
Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
出版信息
J Appl Microbiol. 2001 Sep;91(3):421-6. doi: 10.1046/j.1365-2672.2001.01395.x.
AIMS
To detect and isolate Bacillus anthracis from meat and tissue by rapid and simple procedures.
METHODS AND RESULTS
Bacillus anthracis Pasteur II cells were added to 1 g lymph node and pig meat, which were then cut into small pieces and suspended in PBS. Aliquots were spread on Bacillus cereus selective agar (BCA) plates to isolate B. anthracis cells, and incubated in trypticase soy broth. The enrichment culture was used for nested PCR with B. anthracis specific primers, which were to confirm the presence of B. anthracis chromosomal DNA and the pXO1/pXO2 plasmids.
CONCLUSION
One cell of B. anthracis was detected by nested PCR from 1 g of the samples, and was also isolated on BCA plates according to colony morphology within two days.
SIGNIFICANCE AND IMPACT OF THE STUDY
These results could be useful for detecting animals with latent anthrax, and meat contaminated with B. anthracis, rapidly and simply.
目的
通过快速简便的方法从肉类和组织中检测并分离炭疽芽孢杆菌。
方法与结果
将炭疽芽孢杆菌巴斯德II型菌株加入1克淋巴结和猪肉中，然后切成小块并悬浮于磷酸盐缓冲液（PBS）中。取 aliquots 涂布于蜡样芽孢杆菌选择性琼脂（BCA）平板上以分离炭疽芽孢杆菌细胞，并在胰蛋白胨大豆肉汤中培养。富集培养物用于采用炭疽芽孢杆菌特异性引物的巢式聚合酶链反应（PCR），以确认炭疽芽孢杆菌染色体DNA和pXO1/pXO2质粒的存在。
结论
通过巢式PCR从1克样品中检测到一个炭疽芽孢杆菌细胞，并且在两天内根据菌落形态在BCA平板上也分离到了该菌。
研究的意义和影响
这些结果对于快速简便地检测患有潜伏性炭疽的动物以及被炭疽芽孢杆菌污染的肉类可能有用。
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