Kerebel B, Clergeau-Guerithault S, Forlot P
Ann Microbiol (Paris). 1975 Feb-Mar;126(2):203-29.
Bacterial plaques from the teeth of individuals without caries were prepared for examination under the electron microscope (Ryter and Kellenberger's fixation). Microbial polymorphism within bacterial plaques has previously been usually studied in relation to the age of the plaque. Actually, the presence of polymorphous micro-organisms and variations in microbial density observed in 48 hours old plaques, must be related to other factors. Micro-organisms of the same type, as well as bacteria of different types which adhere together, reveal symbiosis and a competition process within the bacterial plaque, thus leading to changes in metabolism and lysis of many of the bacteria. Disturbances in bacterial cell walls; septal vesicular formation and intracellular structures are observed. Bacteriophages found in the plaques may be the cause of lysis of bacteria. Disturbances in bacterial metabolism could explain the lack of carcinogenicity of micro-organisms, and bacterial antigenic complexes which induce probably useful immunological reactions in the host are revealed by the lack of dental caries.
从无龋齿个体的牙齿上获取菌斑,制备用于电子显微镜检查的样本(采用Ryter和Kellenberger固定法)。此前,通常是结合菌斑的年龄来研究菌斑内的微生物多态性。实际上,在48小时龄的菌斑中观察到的多形微生物的存在以及微生物密度的变化,必定与其他因素有关。同一类型的微生物,以及黏附在一起的不同类型的细菌,在菌斑内呈现出共生和竞争过程,从而导致许多细菌的代谢变化和裂解。观察到细菌细胞壁的紊乱、隔膜泡形成和细胞内结构。菌斑中发现的噬菌体可能是细菌裂解的原因。细菌代谢紊乱可以解释微生物缺乏致癌性,而无龋齿现象则揭示了细菌抗原复合物可能在宿主体内引发有益的免疫反应。