Katrib A, Tak P P, Bertouch J V, Cuello C, McNeil H P, Smeets T J, Kraan M C, Youssef P P
Rheumatology Unit, Prince of Wales Hospital, Inflammation Research Unit, School of Pathology, University of New South Wales, Sydney, Australia.
Rheumatology (Oxford). 2001 Sep;40(9):988-94. doi: 10.1093/rheumatology/40.9.988.
To compare macrophage infiltration and expression of chemokines and matrix metalloproteinases (MMPs) in synovial tissue between patients with early and long-standing rheumatoid arthritis (RA).
Knee synovial biopsies were taken from 22 patients with early (<1 yr) and 22 patients with long-standing (>5 yr) RA and immunostained with antibodies specific for CD68; macrophage inflammatory protein (MIP)-1alpha and monocyte chemoattractant protein (MCP)-1; MMP-1 and -3 and the tissue inhibitors of metalloproteinases (TIMP)-l and -2. Immunostaining was quantified using a colour video image analysis system.
CD68+ macrophage infiltration and the expression of MIP-1alpha, MCP-1, MMP-1, MMP-3, TIMP-1, and TIMP-2 were observed in synovial tissue of patients with early RA. In long-standing RA, there was a further increase in CD68+ macrophage infiltration and MIP-1alpha expression in the synovial lining layer. CD68 expression correlated with MIP-1alpha (R=0.39, P=0.01), but not with MCP-1 expression.
Macrophage accumulation, and the expression of chemokines and MMPs in synovial tissue occur in early RA. Targeting chemokines which play a role in the migration of macrophages into the joints may be of therapeutic benefit in RA patients.
比较早期和长期类风湿关节炎（RA）患者滑膜组织中巨噬细胞浸润以及趋化因子和基质金属蛋白酶（MMPs）的表达情况。
对22例早期（<1年）和22例长期（>5年）RA患者进行膝关节滑膜活检，并用针对CD68、巨噬细胞炎性蛋白（MIP）-1α、单核细胞趋化蛋白（MCP）-1、MMP-1、MMP-3以及金属蛋白酶组织抑制剂（TIMP）-1和TIMP-2的抗体进行免疫染色。使用彩色视频图像分析系统对免疫染色进行定量分析。
在早期RA患者的滑膜组织中观察到CD68 +巨噬细胞浸润以及MIP-1α、MCP-1、MMP-1、MMP-3、TIMP-1和TIMP-2的表达。在长期RA中，滑膜衬里层中CD68 +巨噬细胞浸润和MIP-1α表达进一步增加。CD68表达与MIP-1α相关（R = 0.39，P = 0.01），但与MCP-1表达无关。
早期RA患者滑膜组织中存在巨噬细胞积聚以及趋化因子和MMPs的表达。针对在巨噬细胞向关节迁移中起作用的趋化因子进行靶向治疗可能对RA患者有益。
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