荧光共振能量转移染料核苷酸终止子：一种用于高通量DNA测序的新合成方法。
Fluorescence resonance energy transfer dye nucleotide terminators: a new synthetic approach for high-throughout DNA sequencing.
作者信息
Nampalli S, Khot M, Nelson J R, Flick P K, Fuller C W, Kumar S
机构信息
Amersham Pharmacia Biotech, Piscataway, New Jersey 08855, USA.
出版信息
Nucleosides Nucleotides Nucleic Acids. 2001 Apr-Jul;20(4-7):361-7. doi: 10.1081/NCN-100002307.
Fluorescence resonance energy transfer (FRET) based dye-nucleotide terminators (10-13) were designed, synthesized, and formulated with Thermo Sequenase II DNA polymerase into a robust kit for high throughput DNA sequencing. The key energy transfer (ET) rigid and linear linker (2), required for the syntheses of energy transfer cassettes (6-9) was synthesized via Heck coupling reaction on t-Boc-L-4-iodo-phenylalanine (1) with N-TFA-propargylamine.
基于荧光共振能量转移（FRET）的染料核苷酸终止子（10 - 13）被设计、合成，并与Thermo Sequenase II DNA聚合酶一起配制成用于高通量DNA测序的强大试剂盒。能量转移盒（6 - 9）合成所需的关键能量转移（ET）刚性线性连接子（2）是通过在叔丁氧羰基-L-4-碘苯丙氨酸（1）上与N-三氟乙酰基炔丙胺进行Heck偶联反应合成的。
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