槽毒素，αKTx1.11，一种新型的蝎肽类大电导钙激活钾通道阻滞剂，可区分α和α+β（β1或β4）复合物。
Slotoxin, alphaKTx1.11, a new scorpion peptide blocker of MaxiK channels that differentiates between alpha and alpha+beta (beta1 or beta4) complexes.
作者信息
Garcia-Valdes J, Zamudio F Z, Toro L, Possani L D
机构信息
Department of Molecular Recognition and Structural Biology, Institute of Biotechnology, National Autonomous University of Mexico, Cuernavaca, Mexico.
出版信息
FEBS Lett. 2001 Sep 21;505(3):369-73. doi: 10.1016/s0014-5793(01)02791-0.
A novel peptide from Centruroides noxius Hoffmann scorpion venom was isolated and sequenced. The 37 amino acid peptide belongs to the charybdotoxin sub-family (alphaKTx1) and was numbered member 11. alphaKTx1.11 has 75% sequence identity with iberiotoxin and 54% with charybdotoxin. alphaKTx1.11 revealed specificity for mammalian MaxiK channels (hSlo), thus, was named slotoxin. Slotoxin blocks the MaxiK pore-forming alpha subunit reversibly (K(d)=1.5 nM). Slotoxin association with alpha+beta (beta1 or beta4) channels was approximately 10 times slower than iberiotoxin and charybdotoxin, leading to a lack of effect on alpha+beta4 when tested at 100 nM for 5 min. Thus, slotoxin is a better tool to distinguish MaxiK alpha+beta complexes.
从墨西哥毒蝎（Centruroides noxius Hoffmann）毒液中分离并测序出一种新型肽。这种由37个氨基酸组成的肽属于卡律布狄斯毒素亚家族（alphaKTx1），被编号为成员11，即alphaKTx1.11。alphaKTx1.11与iberiotoxin的序列同一性为75%，与卡律布狄斯毒素的序列同一性为54%。alphaKTx1.11对哺乳动物大电导钙激活钾通道（hSlo）具有特异性，因此被命名为slotoxin。Slotoxin可逆地阻断大电导钙激活钾通道的孔形成α亚基（K(d)=1.5 nM）。Slotoxin与α+β（β1或β4）通道的结合速度比iberiotoxin和卡律布狄斯毒素慢约10倍，在100 nM下测试5分钟时，对α+β4没有影响。因此，slotoxin是区分大电导钙激活钾通道α+β复合物的更好工具。
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