爱泼斯坦-巴尔病毒与伯基特淋巴瘤细胞中免疫球蛋白基因的体细胞超突变
Epstein-Barr virus and the somatic hypermutation of immunoglobulin genes in Burkitt's lymphoma cells.
作者信息
Harris R S, Croom-Carter D S, Rickinson A B, Neuberger M S
机构信息
MRC Laboratory of Molecular Biology, Cambridge CB2 2QH, United Kingdom.
出版信息
J Virol. 2001 Nov;75(21):10488-92. doi: 10.1128/JVI.75.21.10488-10492.2001.
Abstract
It has been suggested that Epstein-Barr virus (EBV) might suppress antibody maturation either by facilitating bypass of the germinal center reaction or by inhibiting hypermutation directly. However, by infecting the Burkitt's lymphoma (BL) cell line Ramos, which hypermutates constitutively and can be considered a transformed analogue of a germinal center B cell, with EBV as well as by transfecting it with selected EBV latency genes, we demonstrate that expression of EBV gene products does not lead to an inhibition of hypermutation. Moreover, we have identified two natural EBV-positive BL cell lines (ELI-BL and BL16) that hypermutate constitutively. Thus, contrary to expectations, EBV gene products do not appear to affect somatic hypermutation.
摘要
有人提出，爱泼斯坦-巴尔病毒（EBV）可能通过促进生发中心反应的旁路或直接抑制高突变来抑制抗体成熟。然而，通过用EBV感染伯基特淋巴瘤（BL）细胞系Ramos（其持续发生高突变，可被视为生发中心B细胞的转化类似物）以及用选定的EBV潜伏基因转染该细胞系，我们证明EBV基因产物的表达不会导致高突变的抑制。此外，我们鉴定出两个持续发生高突变的天然EBV阳性BL细胞系（ELI-BL和BL16）。因此，与预期相反，EBV基因产物似乎不会影响体细胞高突变。
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