重组工程：用于小鼠功能基因组学的强大新工具。

Recombineering: a powerful new tool for mouse functional genomics.

作者信息

Copeland N G, Jenkins N A, Court D L

出版信息

Nat Rev Genet. 2001 Oct;2(10):769-79. doi: 10.1038/35093556.

Abstract

Highly efficient phage-based Escherichia coli homologous recombination systems have recently been developed that enable genomic DNA in bacterial artificial chromosomes to be modified and subcloned, without the need for restriction enzymes or DNA ligases. This new form of chromosome engineering, termed recombinogenic engineering or recombineering, is efficient and greatly decreases the time it takes to create transgenic mouse models by traditional means. Recombineering also facilitates many kinds of genomic experiment that have otherwise been difficult to carry out, and should enhance functional genomic studies by providing better mouse models and a more refined genetic analysis of the mouse genome.

摘要

最近开发了高效的基于噬菌体的大肠杆菌同源重组系统，该系统能够对细菌人工染色体中的基因组DNA进行修饰和亚克隆，而无需限制性内切酶或DNA连接酶。这种新的染色体工程形式，称为重组工程，效率很高，大大缩短了通过传统方法创建转基因小鼠模型所需的时间。重组工程还促进了许多原本难以进行的基因组实验，并且通过提供更好的小鼠模型和对小鼠基因组进行更精细的遗传分析，应该能够加强功能基因组学研究。