Yeboah F A, White D
Department of Molecular Medicine, School of Medical Sciences, University of Science and Technology, Kumasi, Ghana.
Croat Med J. 2001 Oct;42(5):523-6.
To investigate immunohistochemically alphaB-crystallin expression and distribution in biopsy sections of the small intestine of patients with celiac disease, and the relationship, if there is any, between the expression of this heat shock protein and the degree of mucosal atrophy.
Biopsy specimens of 22 subjects (12 celiac patients and 10 "normal" individuals) were investigated by immunoperoxidase screening procedures using monoclonal antibodies to detect the presence of alphaB-crystallin. Sections from each case were stained with hematoxylin and eosin to assess mucosal morphology.
The epithelial cells of the small intestine stained more intensively for alphaB-crystallin in patients with celiac disease than in "normal" patients. The quantity and degree of intensity of immunohistochemically demonstrable alphaB-crystallin and its intracellular distribution in the duodenal mucosa of the patients with celiac disease were closely related to the degree of villus atrophy. There was a strong correlation between the number of patients with celiac disease and percentage staining score of alphaB-crystallin per total score (Pearson's r = 0.754, p = 0.123). The mean percentage score per total score was 20% among the patients with celiac disease. On the other hand, there was a weak negative correlation between the number of normal controls and percentage score per total score (Pearson's r= -0.126, p = 0.437). The mean percentage score per total score was 4.6% among the normal controls. AlphaB-crystallin stained the supra-nuclear region of the enterocytes with a 75% sensitivity and 71% specificity.
AlphaB-crystallin, a stress response protein, is expressed in the small intestinal mucosa of patients with celiac disease, and the degree of mucosal atrophy compares significantly with the intensity of expression of alphaB-crystallin. We therefore conclude that an inappropriate stress response involving this heat-shock protein within the mucosa itself may be crucial as an initiating event in the architectural derangement of the mucosal damage associated with celiac disease.
通过免疫组织化学方法研究乳糜泻患者小肠活检切片中αB-晶状体蛋白的表达和分布情况，以及这种热休克蛋白的表达与黏膜萎缩程度之间是否存在关联。
使用单克隆抗体通过免疫过氧化物酶筛选程序对22名受试者（12名乳糜泻患者和10名“正常”个体）的活检标本进行检测，以检测αB-晶状体蛋白的存在。对每个病例的切片进行苏木精和伊红染色，以评估黏膜形态。
与“正常”患者相比，乳糜泻患者小肠上皮细胞中αB-晶状体蛋白的染色更强烈。免疫组织化学可检测到的αB-晶状体蛋白的数量和强度程度及其在乳糜泻患者十二指肠黏膜中的细胞内分布与绒毛萎缩程度密切相关。乳糜泻患者数量与αB-晶状体蛋白染色分数占总分的百分比之间存在强相关性（Pearson相关系数r = 0.754，p = 0.123）。乳糜泻患者中该分数占总分的平均百分比为20%。另一方面，正常对照数量与分数占总分的百分比之间存在弱负相关（Pearson相关系数r = -0.126，p = 0.437）。正常对照中该分数占总分的平均百分比为4.6%。αB-晶状体蛋白对肠上皮细胞的核上区域进行染色，灵敏度为75%，特异性为71%。
αB-晶状体蛋白作为一种应激反应蛋白，在乳糜泻患者的小肠黏膜中表达，且黏膜萎缩程度与αB-晶状体蛋白的表达强度有显著相关性。因此，我们得出结论，黏膜自身涉及这种热休克蛋白的不适当应激反应可能是乳糜泻相关黏膜损伤结构紊乱的关键起始事件。
