Taguchi K, Saitoh M, Arai Y, Momose K, Ogawa Y, Yasuda S, Miyata K
Pharmacology Laboratories, Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co. Ltd., Tsukuba-shi, Ibaraki, Japan.
Cytokine. 2001 Sep 7;15(5):241-9. doi: 10.1006/cyto.2001.0928.
The effects of interleukin-11 (IL-11) and thrombopoietin (TPO) on murine megakaryocytopoiesis were studied using a serum-free culture system. Acting alone, both IL-11 and TPO increased the number of acetylcholinesterase (AchE)(+)cells (megakaryocytes), the latter being more potent than the former. TPO, but not IL-11, increased the mean AchE activity per megakaryocyte (AchE activity/megakaryocyte). TPO increased both the number of megakaryocytes with high ploidy, and of those with low ploidy. In contrast, IL-11 increased only the number of megakaryocytes with high ploidy. The effect of TPO on megakaryocyte ploidy was stronger than that of IL-11. Both IL-11 and TPO increased the proportion of large megakaryocytes, but the latter was more potent than the former. While the stimulatory effects of IL-11 and TPO on the number of megakaryocytes were enhanced by IL-3 or stem cell factor (SCF), synergism of IL-11 or TPO with IL-3 or SCF in stimulating AchE activity/megakaryocyte was inconsistent. IL-11 and TPO stimulated the formation of colony-forming units of megakaryocyte in the presence of IL-3, but not alone, with similar maximum colony numbers for both cytokines. Our findings thus demonstrate that IL-11 principally stimulates megakaryocyte maturation rather than the proliferation of megakaryocytes, whereas TPO stimulates both.
利用无血清培养系统研究了白细胞介素-11(IL-11)和血小板生成素(TPO)对小鼠巨核细胞生成的影响。单独作用时,IL-11和TPO均可增加乙酰胆碱酯酶(AchE)阳性细胞(巨核细胞)的数量,其中TPO的作用更强。TPO可增加每个巨核细胞的平均AchE活性(AchE活性/巨核细胞),而IL-11则无此作用。TPO可增加高倍体巨核细胞和低倍体巨核细胞的数量。相比之下,IL-11仅增加高倍体巨核细胞的数量。TPO对巨核细胞倍性的影响强于IL-11。IL-11和TPO均可增加大型巨核细胞的比例,但TPO的作用更强。虽然IL-3或干细胞因子(SCF)可增强IL-11和TPO对巨核细胞数量的刺激作用,但IL-11或TPO与IL-3或SCF在刺激每个巨核细胞的AchE活性方面的协同作用并不一致。在有IL-3存在的情况下,IL-11和TPO可刺激巨核细胞集落形成单位的形成,但单独作用时则无此作用,两种细胞因子的最大集落数相似。因此,我们的研究结果表明,IL-11主要刺激巨核细胞成熟而非巨核细胞增殖,而TPO则同时刺激两者。