Shao Z, Shen Z
Department of Surgery, Molecular Biology Lab, Cancer Hospital/Cancer Institute, Shanghai Medical University, Shanghai 200032.
Zhonghua Yi Xue Za Zhi. 1999 Mar;79(3):214-6.
To investigate the effect of mammary myoepithelial cell on breast cancer cell infiltration.
By immunohistochemistry, Western Blot, Northern Blot and Matrigel invasion experiments, we detected the maspin in breast cancer cell and myoepithelial cell, and studied the machanism of breast cancer cell invasion inhibition by myoepithelial cell.
Histologically, ducts and ductal carcinoma in situ (DCIS) were surrounded by an intact circumferential layer of myoepithelial cells. In invasive carcinoma, the myoepithelial cell layer appeared disrupted. In matrigel invasion experiments, myoepithel cell and its condition medium could block the ER-negative breast carcinoma cell line MDA-MB-231 cell invasion. The proteinase inhibitor most dramatically up-regulated in myoepithelial cells was the tumor suppressor maspin which was only highly expressed in myoepithelial cells, not in breast cancer cells.
Mammary myoepithelial cells could inhibit tumor cell invasion, suggesting that myoepithelial cell might have antitumor effect in the regulation of breast cancer development and progression in vivo.
探讨乳腺肌上皮细胞对乳腺癌细胞浸润的影响。
通过免疫组织化学、蛋白质免疫印迹法、Northern印迹法和基质胶侵袭实验，检测乳腺癌细胞和肌上皮细胞中的maspin，并研究肌上皮细胞抑制乳腺癌细胞侵袭的机制。
组织学上，导管和原位导管癌（DCIS）被完整的肌上皮细胞环形层包围。在浸润性癌中，肌上皮细胞层出现破坏。在基质胶侵袭实验中，肌上皮细胞及其条件培养基可阻断雌激素受体阴性的乳腺癌细胞系MDA-MB-231细胞的侵袭。在肌上皮细胞中上调最显著的蛋白酶抑制剂是肿瘤抑制因子maspin，其仅在肌上皮细胞中高表达，而在乳腺癌细胞中不表达。
乳腺肌上皮细胞可抑制肿瘤细胞侵袭，提示肌上皮细胞在体内乳腺癌发生发展的调控中可能具有抗肿瘤作用。
