电磁脉冲对犬外周淋巴细胞的作用及机制
Effect and mechanism of electronic magnetic pulse on peripheral lymphocytes in dogs.
作者信息
Cui Y, Yang H, Gao Y, Cui X, Xiong C, Gao L, Wang D
机构信息
Department of Experimental Pathology, Institute of Radiation Medicine, Beijing 100850, China.
出版信息
Chin Med J (Engl). 2001 Oct;114(10):1019-21.
OBJECTIVE
To study the effects of electronic magnetic pulse (EMP) on peripheral lymphocytes in dogs and to explore the mechanisms of the biological effects of EMP.
METHODS
T, TH and TS lymphocytes were estimated by acid phosphatase cytochemistry. Apoptotic lymphocytes and Bax and Bcl-2 proteins related to apoptosis were observed with in situ terminal labeling and immunocytochemistry.
RESULTS
Peripheral T lymphocyte subpopulations decreased obviously after EMP irradiation with (2-12) x 10(4) V/m. Apoptotic percentages of lymphocytes increased with the elevation of EMP doses. Ten days after different intensity radiation, the Bax protein was found to be elevated in accord with the peak value of lymphocyte apoptosis. However, Bcl-2 protein decreased obviously.
CONCLUSION
A definite field intensity EMP could induce injury to lymphocytes. Apoptosis induced by EMP is one of the main causes of peripheral lymphocyte death and leads to immunosuppression of the body. These results suggest that people should pay more attention to the injury caused by EMP, especially to the immunological functions of the body.
目的
研究电磁脉冲（EMP）对犬外周血淋巴细胞的影响，探讨EMP生物学效应的机制。
方法
采用酸性磷酸酶细胞化学法检测T、TH和TS淋巴细胞。运用原位末端标记法和免疫细胞化学法观察凋亡淋巴细胞以及与凋亡相关的Bax和Bcl-2蛋白。
结果
用（2-12）×10⁴V/m的EMP照射后，外周血T淋巴细胞亚群明显减少。淋巴细胞凋亡率随EMP剂量升高而增加。不同强度辐射10天后，发现Bax蛋白升高，与淋巴细胞凋亡峰值一致。然而，Bcl-2蛋白明显降低。
结论
一定场强的EMP可诱导淋巴细胞损伤。EMP诱导的凋亡是外周血淋巴细胞死亡的主要原因之一，导致机体免疫抑制。这些结果提示人们应更加关注EMP造成的损伤，尤其是对机体免疫功能的影响。
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