del Mar Cabrera M, Valle J, Pajares J M, Romero I, Zomeño M, Maté J
Department of Gastroenterology, Hospital de la Princesa C/Diego de León 62, 28006 Madrid, Spain.
Hepatogastroenterology. 2001 Sep-Oct;48(41):1316-20.
BACKGROUND/AIMS: The presence of natural killer cells in the colon mucosa of patients with ulcerative colitis has not been studied, therefore, the study was designed to investigate the densities of cells expressing CD3+, CD4+, CD8+, CD56+ and the new CD94+ in colon mucosa of active and inactive ulcerative colitis patients.
Twenty ulcerative colitis patients, 10 with active disease and 10 with inactive disease, and 10 subjects with a histologically normal rectal mucosa were used as patients and controls. Additionally, a subgroup of 6 patients with active proctitis has been studied. Two biopsy specimens from rectal mucosa were taken for all patients and controls. Two biopsy specimens of proximal colon mucosa of the subgroup of 6 patients were also taken. One biopsy was processed for immunohistochemical studies and another for histologic study.
The densities of CD3+, CD16+, CD56+ and CD 94+ were significantly increased in active ulcerative colitis patients compared to inactive subjects (P < 0.001). The increase in the CD4+ and CD8+ was not statistically significant. Patients with inactive ulcerative colitis also presented increased numbers of CD3+, CD56+ and CD94+ cells compared to controls (P < 0.001). In the subgroup of proctitis, the densities of cells expressing all the antigens were significantly lower in the normal mucosa compared to the affected colon (P < 0.001). No differences in the number of lamina propria DC1a+ cells between patients and controls were found.
These findings suggest that natural killer cells are increased in active ulcerative colitis subjects and that the affected mucosa of ulcerative colitis patients with inactive disease is in state of "latent" inflammation. On the other hand, the normal looking mucosa from active ulcerative colitis patients does not differ from the mucosa of control subjects. Therefore, it seems that in ulcerative colitis the immunological alterations are limited to the affected mucosa.
背景/目的：溃疡性结肠炎患者结肠黏膜中自然杀伤细胞的存在情况尚未得到研究，因此，本研究旨在调查活动期和非活动期溃疡性结肠炎患者结肠黏膜中表达CD3 +、CD4 +、CD8 +、CD56 +和新发现的CD94 +的细胞密度。
选取20例溃疡性结肠炎患者，其中10例为活动期患者，10例为非活动期患者，另选取10例直肠黏膜组织学正常的受试者作为对照。此外，还对6例活动期直肠炎患者组成的亚组进行了研究。所有患者和对照均取自直肠黏膜的两份活检标本。6例患者亚组的近端结肠黏膜也取了两份活检标本。一份活检标本用于免疫组织化学研究，另一份用于组织学研究。
与非活动期受试者相比，活动期溃疡性结肠炎患者的CD3 +、CD16 +、CD56 +和CD94 +密度显著增加（P < 0.001）。CD4 +和CD8 +的增加无统计学意义。与对照组相比，非活动期溃疡性结肠炎患者的CD3 +、CD56 +和CD94 +细胞数量也有所增加（P < 0.001）。在直肠炎亚组中，正常黏膜中表达所有抗原的细胞密度显著低于受累结肠（P < 0.001）。患者和对照组之间固有层DC1a +细胞数量无差异。
这些发现表明，活动期溃疡性结肠炎患者体内自然杀伤细胞增加，非活动期溃疡性结肠炎患者的受累黏膜处于“潜伏”炎症状态。另一方面，活动期溃疡性结肠炎患者看似正常的黏膜与对照受试者的黏膜并无差异。因此，在溃疡性结肠炎中，免疫改变似乎仅限于受累黏膜。
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