Oliver M, Garcia-Mas J, Cardús M, Pueyo N, López-Sesé A L, Arroyo M, Gómez-Paniagua H, Arús P, de Vicente M C
Institut de Recerca i Tecnologia Agroalimentàries, Departament de Genètica Vegetal, Cabrils, Barcelona, Spain.
Genome. 2001 Oct;44(5):836-45.
A map of melon (Cucumis melo L.) with 411 markers (234 RFLPs, 94 AFLPs, 47 RAPDs, 29 SSRs, five inter-SSRs, and two isozymes) and one morphological trait (carpel number) was constructed using the F2 progeny of a cross between the Korean accession P1161375 and the Spanish melon type 'Pinyonet Piel de Sapo'. RFLPs were obtained using 212 probes from different genomic and cDNA melon libraries, including 16 Arabidopsis ESTs, 13 Cucumis known genes, and three resistant gene homologues. Most loci (391) mapped to 12 major linkage groups, spanning a total genetic distance of 1197 cM, with an average map interval of 3 cM/marker. The remaining 21 loci (six RAPDs and 15 AFLPs) were not linked. A majority (66%) of the markers were codominant (RFLPs, SSRs, and isozymes), making them easily transferable to other melon crosses. Such markers can be used as a reference, to merge other melon and cucumber maps already constructed. Indeed, some of them (23 SSRs, 14 RFLPs, one isozyme, and one morphological trait) could act as anchor points with other published cucurbit maps.
利用韩国种质P1161375与西班牙甜瓜品种“Pinyonet Piel de Sapo”杂交的F2后代构建了一张甜瓜（黄瓜属甜瓜种）图谱，该图谱包含411个标记（234个限制性片段长度多态性标记、94个扩增片段长度多态性标记、47个随机扩增多态性DNA标记、29个简单序列重复标记、5个简单序列重复区间标记和2个同工酶标记）以及一个形态性状（心皮数）。使用来自不同甜瓜基因组文库和cDNA文库的212个探针获得限制性片段长度多态性标记，其中包括16个拟南芥表达序列标签、13个已知的黄瓜属基因和3个抗性基因同源物。大多数位点（391个）定位到12个主要连锁群上，总遗传距离为1197厘摩，平均图距为3厘摩/标记。其余21个位点（6个随机扩增多态性DNA标记和15个扩增片段长度多态性标记）未连锁。大多数（66%）标记为共显性（限制性片段长度多态性标记、简单序列重复标记和同工酶标记），这使得它们易于转移到其他甜瓜杂交组合中。此类标记可作为参考，用于整合已构建的其他甜瓜和黄瓜图谱。实际上，其中一些标记（23个简单序列重复标记、14个限制性片段长度多态性标记、1个同工酶标记和1个形态性状）可作为与其他已发表的葫芦科图谱的锚定标记。
Zotero 插件