Flk-1在小鼠造血干细胞中的作用。
Role of Flk-1 in mouse hematopoietic stem cells.
作者信息
Haruta H, Nagata Y, Todokoro K
机构信息
Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN), 3-1 Koyadai, Ibaraki 305-0074, Tsukuba, Japan.
出版信息
FEBS Lett. 2001 Oct 19;507(1):45-8. doi: 10.1016/s0014-5793(01)02921-0.
It was reported that human hematopoietic stem cells in bone marrow were restricted to the CD34(+)KDR(+) cell fraction. We found that expression levels of Flk-1, a mouse homologue of KDR, were low or undetectable in mouse Lin(-)c-Kit(+)Sca-1(+)CD34(low/-) cells as well as Hoechst33342(-) cells (side population), which have long-term reconstitution capacity. Furthermore, neither Flk-1(+)CD34(low/-) cells nor Flk-1(+)CD34(+) cells had long-term reconstitution capacity in mouse. Taken together with other observations using Flk-1-deficient mice, these results indicate that Flk-1 is essential for the development of hematopoietic stem cells in embryo but not for the function of hematopoietic stem cells in adult mouse bone marrow.
据报道，骨髓中的人类造血干细胞局限于CD34(+)KDR(+)细胞亚群。我们发现，KDR的小鼠同源物Flk-1在具有长期重建能力的小鼠Lin(-)c-Kit(+)Sca-1(+)CD34(low/-)细胞以及Hoechst33342(-)细胞（侧群细胞）中的表达水平很低或无法检测到。此外，Flk-1(+)CD34(low/-)细胞和Flk-1(+)CD34(+)细胞在小鼠中均无长期重建能力。结合使用Flk-1缺陷小鼠的其他观察结果，这些结果表明，Flk-1对胚胎期造血干细胞的发育至关重要，但对成年小鼠骨髓中造血干细胞的功能并非必需。
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