Serrani R E, Mujica G, Gioia I A, Corchs J L
Department of Physiology, School of Medicine, National University of Rosario, Santa Fe, Argentina.
Acta Physiol Pharmacol Bulg. 2000;25(3-4):71-4.
Neonatal red cells (umbilical cord blood) were in vitro incubated in isotonic media (thiocyanate as predominant anion). This experimental condition was selected as it was known that this chaotropic anion induced activation of Na+-H+ exchange. The transport amiloride-sensitive mechanism, that decreased the acidic intracellular pH change occurring in this medium, would correspond to Na+-H+ exchange (NHE1 isoform). However, the Na+-Li+ exchange, also determined in the cells in the above-mentioned medium was not affected by amiloride. The present data suggest that an amiloride insensitive Na+-H+ exchange isoform would express Na+-Li+ countertransport in these cells.
将新生儿红细胞（脐带血）在等渗培养基（硫氰酸盐作为主要阴离子）中进行体外培养。选择这种实验条件是因为已知这种离液序列高的阴离子会诱导Na⁺-H⁺交换的激活。降低在此培养基中发生的细胞内酸性pH变化的氨氯地平敏感转运机制，将对应于Na⁺-H⁺交换（NHE1亚型）。然而，在上述培养基中的细胞中测定的Na⁺-Li⁺交换不受氨氯地平的影响。目前的数据表明，一种氨氯地平不敏感的Na⁺-H⁺交换亚型将在这些细胞中表达Na⁺-Li⁺逆向转运。
Zotero 插件