Prystay L, Gosselin M, Banks P
PerkinElmer Life Sciences, Montreal, Quebec, Canada.
J Biomol Screen. 2001 Jun;6(3):141-50. doi: 10.1177/108705710100600304.
A simple mathematical model (the FP K(d) model) is used to generate the dissociation equilibrium constant (K(d)) for G protein-coupled receptor-ligand binding measured using fluorescence polarization (FP) saturation curve analysis. The model generates data that may be analyzed by the method of Scatchard. The validity of the FP K(d) model is proven in six model systems in which the modeled K(d) values are within a factor of 5 of inhibitory equilibrium constant values obtained from radioligand competition assays.
一个简单的数学模型（FP K(d)模型）用于生成通过荧光偏振（FP）饱和曲线分析测量的G蛋白偶联受体-配体结合的解离平衡常数（K(d)）。该模型生成的数据可用Scatchard方法进行分析。FP K(d)模型的有效性在六个模型系统中得到证实，其中建模的K(d)值在从放射性配体竞争试验获得的抑制平衡常数的5倍范围内。
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