肝脏组织中的胸苷磷酸化酶活性及其与肝细胞癌多灶性发生的相关性。

Thymidine phosphorylase activity in liver tissue and its correlation with multifocal occurrence of hepatocellular carcinomas.

作者信息

Ikeguchi M, Sakatani T, Ueda T, Hirooka Y, Kaibara N

机构信息

First Department of Surgery, Faculty of Medicine, Tottori University, Nishi-cho 86-1, Yonago 683-8503, Japan.

出版信息

In Vivo. 2001 Jul-Aug;15(4):265-70.

PMID:11695216

Abstract

BACKGROUND

In hepatocellular carcinoma (HCC), new tumors develop in the residual liver within a few years after hepatectomy. However, the biological risk factors of multifocal occurrence of cancers remains unclear. In this study, the thymidine phosphorylase (TP) activity, which is known as an angiogenic factor, of cancerous and non-cancerous liver tissues in HCC was analyzed to determine its suitability as a biological marker of the multifocal occurrence of HCCs.

MATERIALS AND METHODS

Fresh tissues (tumor: HCC and adjacent liver tissue: N-HCC) from 63 patients with HCC and normal liver tissues (NL) from 6 patients without HCC were obtained. The TP activities of the tissues were analyzed by an enzyme-linked immunosorbent assay (ELISA).

RESULTS

The mean TP activity of 63 HCCs (136 U/mg protein) was higher than that of 63 N-HCCs (81 U/mg protein) and that of 6 NLs (47 U/mg protein, p < 0.001). Multifocal occurrence of HCCs were detected in 17 patients. In these 17 patients, the mean TP activity of HCCs (145 U/mg protein) was not different from that of HCCs from the remaining 46 patients (133 U/mg protein, p = 0.272), however the mean TP activity of N-HCCs (110 U/mg protein) was significantly higher than that of N-HCCs from the remaining 46 patients (71 U/mg protein, p = 0.038). Moreover, only a high TP activity of N-HCCs was detected as a significant risk factor of multifocal occurrence of HCCs.

CONCLUSION

Patients who have tumors with high TP activity in the non-cancerous livers may have a risk of multifocal occurrence of HCCs in the residual liver.

摘要

背景

在肝细胞癌（HCC）中，肝切除术后几年内残余肝脏会出现新的肿瘤。然而，癌症多灶性发生的生物学危险因素仍不清楚。在本研究中，分析了HCC中癌组织和非癌组织的胸苷磷酸化酶（TP）活性，TP是一种血管生成因子，以确定其作为HCC多灶性发生生物学标志物的适用性。

材料与方法

获取63例HCC患者的新鲜组织（肿瘤：HCC和邻近肝组织：N-HCC）以及6例非HCC患者的正常肝组织（NL）。通过酶联免疫吸附测定（ELISA）分析组织的TP活性。

结果

63例HCC的平均TP活性（136 U/mg蛋白）高于63例N-HCC（81 U/mg蛋白）和6例NL（47 U/mg蛋白，p<0.001）。在17例患者中检测到HCC多灶性发生。在这17例患者中，HCC的平均TP活性（145 U/mg蛋白）与其余46例患者的HCC（133 U/mg蛋白，p = 0.272）无差异，然而N-HCC的平均TP活性（110 U/mg蛋白）显著高于其余46例患者的N-HCC（71 U/mg蛋白，p = 0.038）。此外，仅检测到N-HCC的高TP活性是HCC多灶性发生的显著危险因素。