着丝粒蛋白B对雄性小鼠减数分裂染色体分离并不关键。

CENP-B is not critical for meiotic chromosome segregation in male mice.

作者信息

Tomascik-Cheeseman Lisa, Marchetti Francesco, Lowe Xiu, Shamanski Fay L, Nath Joginder, Pedersen Roger A, Wyrobek Andrew J

机构信息

Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, P.O. Box 808 L-448, Livermore, CA 94550, USA.

出版信息

Mutat Res. 2002 Jan 15;513(1-2):197-203. doi: 10.1016/s1383-5718(01)00315-1.

DOI:10.1016/s1383-5718(01)00315-1

PMID:11719105

Abstract

Centromere protein B (CENP-B) is a constitutive protein that binds to a highly conserved 17bp motif located at most mammalian centromeres. To determine whether disruption of this gene affects chromosome segregation in male germ cells, we evaluated the frequencies of disomic and diploid sperm in CENP-B heterozygous and homozygous null mice using the mouse epididymal sperm aneuploidy (m-ESA) assay, a multicolor FISH method with probes for chromosomes X, Y and 8. The specificity and sensitivity of the m-ESA assay was demonstrated using Robertsonian (2.8) translocation heterozygotes as positive controls for sperm aneuploidy. Our results show that the frequencies of disomic and diploid sperm did not differ significantly between CENP-B heterozygous and homozygous null mice (P> or = 0.5) or from 129/Swiss isogenic mice (P> or = 0.5) and B6C3F1 mice (P> or = 0.2). These findings indicate that CENP-B does not have an essential role during chromosome segregation in male meiosis.

摘要

着丝粒蛋白B（CENP - B）是一种组成型蛋白，可与位于大多数哺乳动物着丝粒的高度保守的17bp基序结合。为了确定该基因的破坏是否会影响雄性生殖细胞中的染色体分离，我们使用小鼠附睾精子非整倍体（m - ESA）检测方法（一种使用X、Y和8号染色体探针的多色荧光原位杂交方法），评估了CENP - B杂合和纯合缺失小鼠中二体精子和二倍体精子的频率。使用罗伯逊易位（2.8）杂合子作为精子非整倍体的阳性对照，证明了m - ESA检测方法的特异性和敏感性。我们的结果表明，CENP - B杂合和纯合缺失小鼠之间二体精子和二倍体精子的频率没有显著差异（P≥0.5），与129 / Swiss同基因小鼠（P≥0.5）和B6C3F1小鼠（P≥0.2）相比也没有显著差异。这些发现表明，CENP - B在雄性减数分裂的染色体分离过程中不具有关键作用。