Brokaw L, Hess B W, Rule D C
Department of Animal Science, University of Wyoming, Laramie 82071-3864, USA.
J Anim Sci. 2001 Oct;79(10):2704-12. doi: 10.2527/2001.79102704x.
Nine Angus x Gelbvieh heifers (average BW = 347 +/- 2.8 kg) with ruminal and duodenal cannulas were used in a split-plot designed experiment to determine the effects of soybean oil or corn supplementation on intake, OM, NDF, and N digestibility. Beginning June 8, 1998, heifers continually grazed a 6.5-ha predominantly bromegrass pasture and received one of three treatments: no supplementation (Control); daily supplementation of cracked corn (Corn) at 0.345% of BW; or daily supplementation (0.3% of BW) of a supplement containing cracked corn, corn gluten meal, and soybean oil (12.5% of supplemental DM; Oil). Soybean oil replaced corn on a TDN basis and corn gluten meal was included to provide equal quantities of supplemental TDN and N. Three 23-d periods consisted of 14 d of adaptation followed by 9 d of sample collections. Treatment and sampling period effects were evaluated using orthogonal contrasts. Other than crude fat being greater (P = 0.01) for supplemented heifers, chemical and nutrient composition of masticate samples collected via ruminal evacuation did not differ (P = 0.23 to 0.56) among treatments. Masticate NDF and ADF increased quadratically (P < or = 0.003) and N decreased linearly (P = 0.0001) as the grazing season progressed. Supplementation did not influence (P = 0.37 to 0.83) forage OM intake, total and lower tract OM digestibility, ruminal and total tract NDF digestibility, or total ruminal VFA; however, supplemented heifers had lower ruminal molar proportions of acetate (P = 0.01), higher ruminal molar proportions of butyrate (P = 0.007), and greater quantities of OM digested in the rumen (P = 0.10) and total tract (P = 0.02). As the grazing season progressed, total tract OM and N and ruminal NH3 concentrations and NDF digestibility decreased quadratically (P < or = 0.04). Microbial N flow (P = 0.09) and efficiency (P = 0.04) and postruminal N disappearance (P = 0.02) were greater for Control heifers and declined linearly (P < or = 0.02) as the grazing season advanced. Depressed microbial N flow seemed to be more pronounced for Oil than for the Corn treatment. Although total digestible OM intake increased with supplementation, metabolizable protein supply was reduced in supplemented heifers. Therefore, feeding low levels of supplemental grain with or without soybean oil is an effective strategy to increase dietary energy for cattle grazing high-quality forages, but consideration should be given to reduced supply of metabolizable protein.
选用9头安装了瘤胃和十二指肠瘘管的安格斯×盖尔维杂交小母牛(平均体重=347±2.8千克),进行裂区设计试验,以确定补充大豆油或玉米对采食量、有机物(OM)、中性洗涤纤维(NDF)和氮消化率的影响。从1998年6月8日开始,小母牛持续放牧一片6.5公顷、以雀麦为主的牧场,并接受三种处理之一:不补充(对照);按体重的0.345%每日补充破碎玉米(玉米组);或按体重的0.3%每日补充一种含有破碎玉米、玉米蛋白粉和大豆油(占补充干物质的12.5%;油组)的补充料。大豆油按总可消化养分(TDN)基准替代玉米,并添加玉米蛋白粉以提供等量的补充TDN和氮。三个23天的周期包括14天的适应期,随后是9天的样本采集期。使用正交对比评估处理和采样期效应。除了补充组小母牛的粗脂肪含量更高(P=0.01)外,通过瘤胃排空收集的咀嚼样本的化学和营养成分在各处理间没有差异(P=0.23至0.56)。随着放牧季节的推进,咀嚼样本的NDF和酸性洗涤纤维(ADF)呈二次增加(P≤0.003),氮呈线性下降(P=0.0001)。补充处理对牧草OM采食量、上、下消化道OM消化率、瘤胃和全消化道NDF消化率或瘤胃总挥发性脂肪酸(VFA)没有影响(P=0.37至0.83);然而,补充组小母牛瘤胃乙酸摩尔比例较低(P=0.01),丁酸摩尔比例较高(P=0.007),瘤胃(P=0.10)和全消化道(P=0.02)消化的OM量更多。随着放牧季节的推进,全消化道OM和氮以及瘤胃氨浓度和NDF消化率呈二次下降(P≤0.04)。对照小母牛的微生物氮流量(P=0.09)和效率(P=0.04)以及瘤胃后氮消失量(P=0.02)更高,并随着放牧季节的推进呈线性下降(P≤0.02)。油组小母牛微生物氮流量的降低似乎比玉米组更明显。虽然补充处理后总可消化OM采食量增加,但补充组小母牛的可代谢蛋白质供应减少。因此,给放牧优质牧草的牛饲喂低水平的补充谷物(无论是否添加大豆油)是增加日粮能量的有效策略,但应考虑可代谢蛋白质供应的减少。