通过双重逆转录聚合酶链反应（duplex RT-PCR）鉴别检测传染性胃肠炎病毒和猪流行性腹泻病毒

Differential detection of transmissible gastroenteritis virus and porcine epidemic diarrhea virus by duplex RT-PCR.

作者信息

Kim S Y, Song D S, Park B K

机构信息

Department of Veterinary Medicine Virology Lab, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Suwon, Korea.

出版信息

J Vet Diagn Invest. 2001 Nov;13(6):516-20. doi: 10.1177/104063870101300611.

DOI:10.1177/104063870101300611

PMID:11724144

Abstract

Transmissible gastroenteritis (TGE) and porcine epidemic diarrhea (PED) are highly contagious enteric diseases of piglets. The clinical signs of these diseases are very similar and include watery, yellowish diarrhea. Thus, the effective differential detection of TGE virus and PED virus is required. In the present study, a duplex reverse transcription-polymerase chain reaction (RT-PCR) was established for the differential detection of TGE and PED viruses. The primers were designed for the S gene of each virus. RNA was extracted from the intestines and stool samples that were collected from the swine with diarrhea. The RT-PCR test could detect both TGE and PED viruses with 2 TCID50/200 microl. Among 90 clinical samples, 7 TGE viruses and 2 PED viruses were detected by the duplex RT-PCR. This duplex RT-PCR may be a useful diagnostic method for the rapid, specific, and sensitive differential detection of TGE and PED viruses using clinical samples.

摘要

传染性胃肠炎（TGE）和猪流行性腹泻（PED）是仔猪的高度传染性肠道疾病。这些疾病的临床症状非常相似，包括水样、淡黄色腹泻。因此，需要对TGE病毒和PED病毒进行有效的鉴别检测。在本研究中，建立了一种双重逆转录-聚合酶链反应（RT-PCR）方法用于TGE和PED病毒的鉴别检测。针对每种病毒的S基因设计了引物。从腹泻猪的肠道和粪便样本中提取RNA。RT-PCR试验能够以2个半数组织培养感染剂量（TCID50）/200微升的浓度检测到TGE和PED病毒。在90份临床样本中，通过双重RT-PCR检测到7株TGE病毒和2株PED病毒。这种双重RT-PCR可能是一种利用临床样本快速、特异性和灵敏地鉴别检测TGE和PED病毒的有用诊断方法。