使用AMPFlSTR SGM Plus复合扩增系统进行标准和低拷贝数（LCN）STR DNA分析所产生的图谱特征比较。

A comparison of the characteristics of profiles produced with the AMPFlSTR SGM Plus multiplex system for both standard and low copy number (LCN) STR DNA analysis.

作者信息

Whitaker J P, Cotton E A, Gill P

机构信息

Research and Development, The Forensic Science Service, Trident Court, Solihull Parkway, Birmingham Business Park, Solihull B37 7YN, UK.

出版信息

Forensic Sci Int. 2001 Dec 1;123(2-3):215-23. doi: 10.1016/s0379-0738(01)00557-6.

DOI:10.1016/s0379-0738(01)00557-6

PMID:11728750

Abstract

DNA STR profiles have been generated from 1 ng and low copy number (LCN) templates using 28 and 34 cycles of amplification, respectively. Characteristics which facilitate the interpretation of profiles, such as heterozygous balance, allelic dropout and stutter proportions have been quantified. We demonstrate that a reduction in DNA template coupled with an increase in amplification cycle number produces an increased rate of allelic dropout out which can be correlated to the peak areas of those alleles observed. In addition, the LCN conditions increase the degree of peak area asymmetry observed from heterozygotes and the size range of stutters. Analysis of the data allows us to develop sets of guidelines appropriate for interpreting both single and mixed DNA profiles.

摘要

分别使用28个和34个循环的扩增，从1纳克和低拷贝数（LCN）模板生成了DNA STR图谱。对有助于图谱解释的特征进行了量化，如杂合平衡、等位基因丢失和拖尾比例。我们证明，DNA模板减少与扩增循环数增加会导致等位基因丢失率增加，这与观察到的那些等位基因的峰面积相关。此外，LCN条件增加了杂合子观察到的峰面积不对称程度以及拖尾的大小范围。对数据的分析使我们能够制定适用于解释单个和混合DNA图谱的指南集。