鼠李属植物中槲皮素3 - O - 甲基醚及其他成分的体外抗炎作用。

In vitro anti-inflammatory effects of quercetin 3-O-methyl ether and other constituents from Rhamnus species.

作者信息

Wei B L, Lu C M, Tsao L T, Wang J P, Lin C N

机构信息

National University of Kaohsiung, Kaohsiung, Taiwan, Republic of China.

出版信息

Planta Med. 2001 Nov;67(8):745-7. doi: 10.1055/s-2001-18339.

DOI:10.1055/s-2001-18339

PMID:11731918

Abstract

The anti-inflammatory activities of the isolated flavonoids, quercetin 3-O-methyl ether (1), kaempferol (2), and quercetin (3), of Rhamnus nakaharai, and anthraquinone, frangulin B (4), of Rhamnus formosana, were assessed in vitro by determining their inhibitory effects on the chemical mediators released from mast cells, neutrophils, macrophages, and microglial cells. Compounds 1 - 3 strongly inhibited the release of beta-glucuronidase and lysozyme from rat neutrophils stimulated with formyl-Met-Leu-Phe/cytochalasin B (fMLP/CB). Compound 1 strongly inhibited superoxide anion formation in fMLP/CB or phorbol 12-myristate 13-acetate (PMA)-stimulated rat neutrophils. Compound 1 exhibited potent inhibitory effect on tumor-necrosis factor-alpha ( TNF-alpha) formation in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells while 1 and 4 showed potent inhibitory effects on TNF-alpha formation in LPS/IFN-gamma (interferon-gamma)-stimulated murine microglial cell lines N9.

摘要

通过测定其对肥大细胞、中性粒细胞、巨噬细胞和小胶质细胞释放的化学介质的抑制作用，在体外评估了鼠李（Rhamnus nakaharai）中分离出的黄酮类化合物槲皮素3 - O - 甲基醚（1）、山奈酚（2）和槲皮素（3）以及台湾鼠李（Rhamnus formosana）中的蒽醌类化合物异鼠李素B（4）的抗炎活性。化合物1 - 3强烈抑制了用甲酰 - 甲硫氨酸 - 亮氨酸 - 苯丙氨酸/细胞松弛素B（fMLP/CB）刺激的大鼠中性粒细胞中β - 葡萄糖醛酸酶和溶菌酶的释放。化合物1强烈抑制fMLP/CB或佛波酯12 - 肉豆蔻酸酯13 - 乙酸酯（PMA）刺激的大鼠中性粒细胞中超氧阴离子的形成。化合物1对脂多糖（LPS）刺激的RAW 264.7细胞中肿瘤坏死因子 - α（TNF - α）的形成具有显著抑制作用，而化合物1和4对LPS/γ干扰素（IFN - γ）刺激的小鼠小胶质细胞系N9中TNF - α的形成具有显著抑制作用。