烟草根中两种硝酸还原酶转录本的不同昼夜表达周期。
Different diurnal cycles of expression of two nitrate reductase transcripts in tobacco roots.
作者信息
Wienkoop S, Schlichting R, Ullrich W R, Stöhr C
机构信息
Institut für Botanik, Technische Universität Darmstadt, Schnittspahnstrasse 10, 64287 Darmstadt, Federal Republic of Germany.
出版信息
Protoplasma. 2001;217(1-3):15-9. doi: 10.1007/BF01289408.
In roots and leaves of tobacco (Nicotiana tabacum cv. Samsun) three functional transcripts (3.6 kb, 3.1 kb, and 1.8 kb) were found to at least partly represent nitrate reductase mRNA. With specific probes for the transcripts of the different domains of nitrate reductase it was shown that the smallest transcript was shortened in the region coding for the flavin adenine dinucleotide domain and might be the transcript coding for plasma-membrane-bound nitrate reductase. The expression of the 3.1 kb and 1.8 kb transcripts in roots was differently regulated during the day-night cycle with the maximum amount of the 3.1 kb transcript in the middle and of the 1.8 kb transcript at the end of the light period.
在烟草（Nicotiana tabacum cv. Samsun）的根和叶中，发现三种功能性转录本（3.6 kb、3.1 kb和1.8 kb）至少部分代表硝酸还原酶mRNA。使用针对硝酸还原酶不同结构域转录本的特异性探针表明，最小的转录本在编码黄素腺嘌呤二核苷酸结构域的区域被缩短，可能是编码质膜结合硝酸还原酶的转录本。3.1 kb和1.8 kb转录本在根中的表达在昼夜周期中受到不同调节，3.1 kb转录本在白天中间含量最高，1.8 kb转录本在光照期结束时含量最高。
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