Abou-Elenin Karim, Xydakis Antonios, Hamdy Osama, Economides Panayiotis A, Horton Edward S, Veves Aristidis
Clinical Research Center, Joslin Diabetes Center, Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215, USA.
Microvasc Res. 2002 Jan;63(1):91-5. doi: 10.1006/mvre.2001.2369.
The two main objectives of this study were: (1) to examine the effect of aspirin on the endothelial function in healthy subjects and (2) to examine the effect of deionized water and 5% NaCl as iontophoresis solution vehicles. The skin microcirculation was evaluated at the forearm level of healthy subjects. A laser Doppler scanner was employed to measure vasodilation in response to the iontophoresis of 1% acetylcholine (endothelium-dependent) and 1% sodium nitroprusside (endothelium-independent). In the first experiment, nine healthy subjects were given 500 mg aspirin daily for 3 days. The microvascular reactivity was measured at the beginning and the end of the study. In the second experiment, the response to iontophoresis of acetylcholine and sodium nitroprusside as 1% solutions of deionized water was compared to the responses that were achieved after the iontophoresis of deionized water or 5% NaCl solution. After 3 days of aspirin intake, there were no changes in the vasodilatory response to acetylcholine (endothelium-dependent vasodilation) [81 +/- 11 vs 77 +/- 10 (% of increase over baseline at the beginning vs the end of the study, mean +/- SE), P = NS] or sodium nitroprusside (endothelium-independent vasodilation) (69 +/- 8 vs 64 +/- 12, P = NS). There was also a negligible response after the iontophoresis of 5% NaCl (3 +/- 4) and deionized water (6 +/- 4) in anodal mode (the mode employed for the iontophoresis of acetylcholine). In cathodal mode, employed for the iontophoresis of sodium nitroprusside, the response to 5% NaCl was still negligible but a considerable response was found after the iontophoresis of deionized water. In normal healthy subjects, aspirin administration has no effect on forearm skin microvascular reactivity, including both endothelium-dependent and endothelium-independent vasodilation. In addition, a NaCl solution would be preferable to deionized water as the iontophoresis solution vehicle.
（1）研究阿司匹林对健康受试者内皮功能的影响；（2）研究去离子水和5%氯化钠作为离子导入溶液载体的效果。在健康受试者的前臂水平评估皮肤微循环。使用激光多普勒扫描仪测量对1%乙酰胆碱（内皮依赖性）和1%硝普钠（非内皮依赖性）离子导入的血管舒张反应。在第一个实验中，9名健康受试者每天服用500毫克阿司匹林，持续3天。在研究开始和结束时测量微血管反应性。在第二个实验中，将1%去离子水溶液的乙酰胆碱和硝普钠离子导入反应与去离子水或5%氯化钠溶液离子导入后的反应进行比较。服用阿司匹林3天后，对乙酰胆碱（内皮依赖性血管舒张）的血管舒张反应[研究开始时与结束时相对于基线的增加百分比分别为81±11与77±10，平均值±标准误，P=无显著性差异]或硝普钠（非内皮依赖性血管舒张）（69±8与64±12，P=无显著性差异）没有变化。在阳极模式（用于乙酰胆碱离子导入的模式）下，5%氯化钠（3±4）和去离子水（6±4）离子导入后的反应也可忽略不计。在用于硝普钠离子导入的阴极模式下，对5%氯化钠的反应仍然可忽略不计，但去离子水离子导入后发现有相当大的反应。在正常健康受试者中，服用阿司匹林对前臂皮肤微血管反应性没有影响，包括内皮依赖性和非内皮依赖性血管舒张。此外，作为离子导入溶液载体，氯化钠溶液比去离子水更可取。
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