溶解氧（DO）浓度对昆虫细胞-杆状病毒表达系统生产的重组蛋白糖基化的影响。

The effect of dissolved oxygen (DO) concentration on the glycosylation of recombinant protein produced by the insect cell-baculovirus expression system.

作者信息

Zhang Fuming, Saarinen Mark A, Itle Laura J, Lang Sybil C, Murhammer David W, Linhardt Robert J

机构信息

Department of Chemical and Biochemical Engineering, University of Iowa, Iowa City, Iowa 52242, USA.

出版信息

Biotechnol Bioeng. 2002 Jan 20;77(2):219-24. doi: 10.1002/bit.10131.

DOI:10.1002/bit.10131

PMID:11753929

Abstract

The effect of dissolved oxygen concentration on human secreted alkaline phosphatase (SEAP) glycosylation by the insect cell-baculovirus expression system was investigated in a well-controlled bioreactor. Oligomannose-type N-linked glycans (i.e., Man2 to Man6 and Man3F) were present in SEAP produced by Spodoptera frusiperda Sf-9 (Sf-9) and Trichoplusia ni BTI-Tn-5B1-4 (Tn-5B1-4) insect cell lines. The relative amounts of the most highly processed glycans (i.e., Man3F and Man2 in the SEAP from Sf-9 and Tn-5B1-4 cells, respectively) were significantly higher at 50% of air saturation than at either 10% or 190% of air saturation. That is, glycan processing was inhibited at both low and high dissolved oxygen concentrations.

摘要

在一个严格控制的生物反应器中，研究了溶解氧浓度对昆虫细胞-杆状病毒表达系统产生的人分泌型碱性磷酸酶（SEAP）糖基化的影响。寡甘露糖型N-连接聚糖（即Man2至Man6和Man3F）存在于草地贪夜蛾Sf-9（Sf-9）和粉纹夜蛾BTI-Tn-5B1-4（Tn-5B1-4）昆虫细胞系产生的SEAP中。在空气饱和度为50%时，加工程度最高的聚糖（即分别来自Sf-9和Tn-5B1-4细胞的SEAP中的Man3F和Man2）的相对含量显著高于空气饱和度为10%或190%时。也就是说，在低溶解氧浓度和高溶解氧浓度下，聚糖加工均受到抑制。