胃泌素原加工在7B2和PC2基因敲除动物中存在差异：7B2的作用独立于其对PC2的作用。

Progastrin processing differs in 7B2 and PC2 knockout animals: a role for 7B2 independent of action on PC2.

作者信息

Rehfeld Jens F, Lindberg Iris, Friis-Hansen Lennart

机构信息

Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.

出版信息

FEBS Lett. 2002 Jan 2;510(1-2):89-93. doi: 10.1016/s0014-5793(01)03234-3.

DOI:10.1016/s0014-5793(01)03234-3

PMID:11755537

Abstract

Cellular synthesis of neuroendocrine peptides requires prohormone convertases (PCs). In order to determine the role of PC2 for gastrin synthesis, we examined antral extracts from mice lacking PC2 or its chaperone, 7B2. The overall concentrations of precursors and alpha-amidated gastrins were similar in all mice. Chromatography, however, revealed that while the K(53)-K(54) site was almost fully cleaved in controls and half cleaved in PC2 null mice, only 23% was cleaved in 7B2 null mice. The results show that PC2 and 7B2 both are required for synthesis of the main form of gastrin (gastrin-17), and that 7B2 exhibits effects beyond PC2-mediated cleavages.

摘要

神经内分泌肽的细胞合成需要激素原转化酶（PCs）。为了确定PC2在胃泌素合成中的作用，我们检测了缺乏PC2或其伴侣蛋白7B2的小鼠的胃窦提取物。所有小鼠中前体和α-酰胺化胃泌素的总体浓度相似。然而，色谱分析显示，虽然K(53)-K(54)位点在对照组中几乎完全裂解，在PC2基因敲除小鼠中裂解一半，但在7B2基因敲除小鼠中只有23%被裂解。结果表明，PC2和7B2都是胃泌素主要形式（胃泌素-17）合成所必需的，并且7B2表现出超出PC2介导裂解的作用。